CSDR Coupling with Exo III for Ultrasensitive Electrochemistry Determination of miR-145
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Published:2023-02-27
Issue:5
Volume:28
Page:2208
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ISSN:1420-3049
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Container-title:Molecules
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language:en
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Short-container-title:Molecules
Author:
Zhang Moli1,
Yang Yang1,
Xin Lingyi2,
Zhang Hua3,
Wu Lun3,
Zhu Jun3,
Zhu Jing2,
Liu Shiyun3,
Wang Zhaohui1,
Chen Qinhua1ORCID,
Yang Guangyi2
Affiliation:
1. Shenzhen Bao’an Authentic TCM Therapy Hospital, Shenzhen 518102, China
2. Shenzhen Bao’an Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen 518000, China
3. Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 430345, China
Abstract
Recently, miRNAs have become a promising biomarker for disease diagnostics. miRNA-145 is closely related to strokes. The accuracy determination of miRNA-145 (miR-145) in stroke patients still remains challenging due to its heterogeneity and low abundance, as well as the complexity of the blood matrix. In this work, we developed a novel electrochemical miRNA-145 biosensor via subtly coupling the cascade strand displacement reaction (CSDR), exonuclease III (Exo III), and magnetic nanoparticles (MNPs). The developed electrochemical biosensor can quantitatively detect miRNA-145 ranging from 1 × 102 to 1 × 106 aM with a detection limit as low down as 100 aM. This biosensor also exhibits excellent specificity to distinguish similar miRNA sequences even with single-base differences. It has been successfully applied to distinguish healthy people from stroke patients. The results of this biosensor are consistent with the results of the reverse transcription quantitative polymerase chain reaction (RT-qPCR). The proposed electrochemical biosensor has great potential applications for biomedical research on and clinical diagnosis of strokes.
Funder
Sanming Project of Medicine in Shenzhen
National Natural Science Foundation of China
Guangdong Provincial Bureau of traditional Chinese Medicine
Shenzhen Science and Technology Innovation Commission
Bao’an Authentic TCM Therapy Hospital
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
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