Natamycin Has an Inhibitory Effect on Neofusicoccum parvum, the Pathogen of Chestnuts

Author:

Gou Lin-Jing12,Liu Tian-Tian12,Zeng Qi12,Dong Wan-Rong1,Wang Lu1,Long Sha1,Su Jiang-Tao1ORCID,Chen Yu-Xin12ORCID,Zhou Gao123ORCID

Affiliation:

1. Hubei Key Laboratory of Industrial Microbiology, Key Laboratory of Fermentation Engineering (Ministry of Education), Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Hubei University of Technology, Wuhan 430068, China

2. National “111” Center for Cellular Regulation and Molecular Pharmaceutics, School of Biological Engineering and Food, Hubei University of Technology, Wuhan 430068, China

3. Post-Doctoral Research Center of Mayinglong Pharmaceutical Group Co., Ltd., Wuhan 430064, China

Abstract

This research aimed to investigate natamycin’s antifungal effect and its mechanism against the chestnut pathogen Neofusicoccum parvum. Natamycin’s inhibitory effects on N. parvum were investigated using a drug-containing plate culture method and an in vivo assay in chestnuts and shell buckets. The antifungal mechanism of action of natamycin on N. parvum was investigated by conducting staining experiments of the fungal cell wall and cell membrane. Natamycin had a minimum inhibitory concentration (MIC) of 100 μg/mL and a minimum fungicidal concentration (MFC) of 200 μg/mL against N. parvum. At five times the MFC, natamycin had a strong antifungal effect on chestnuts in vivo, and it effectively reduced morbidity and extended the storage period. The cell membrane was the primary target of natamycin action against N. parvum. Natamycin inhibits ergosterol synthesis, disrupts cell membranes, and causes intracellular protein, nucleic acid, and other macromolecule leakages. Furthermore, natamycin can cause oxidative damage to the fungus, as evidenced by decreased superoxide dismutase and catalase enzyme activity. Natamycin exerts a strong antifungal effect on the pathogenic fungus N. parvum from chestnuts, mainly through the disruption of fungal cell membranes.

Funder

National Natural Science Foundation of China

Hubei Provincial Education Department Natural Science Foundation

Collaborative Grant-in-Aid of the HBUT National “111” Center for Cellular Regulation and Molecular Pharmaceutics

Doctoral Start-up Foundation of Hubei University of Technology

Publisher

MDPI AG

Subject

Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science

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