Fluorescence Turn-Off Ligand for Parallel G-Quadruplexes-Reference-Cited by-同舟云学术

Fluorescence Turn-Off Ligand for Parallel G-Quadruplexes

Published:2024-08-18 Issue:16 Volume:29 Page:3907
ISSN:1420-3049
Container-title:Molecules
language:en
Short-container-title:Molecules

Author:

Nowak-Karnowska Joanna¹^ORCID,Głuszyńska Agata¹^ORCID,Kosman Joanna¹²^ORCID,Dembska Anna¹^ORCID

Affiliation:

1. Department of Bioanalytical Chemistry, Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland

2. Laboratory of Molecular Assays and Imaging, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland

Abstract

Parallel-stranded G-quadruplex structures are found to be common in the human promoter sequences. We tested highly fluorescent 9-methoxyluminarine ligand (9-MeLM) binding interactions with different parallel G-quadruplexes DNA by spectroscopic methods such as fluorescence and circular dichroism (CD) titration as well as UV melting profiles. The results showed that the studied 9-MeLM ligand interacted with the intramolecular parallel G-quadruplexes (G4s) with similar affinity. The binding constants of 9-methoxyluminarine with different parallel G4s were determined. The studies upon oligonucleotides with different flanking sequences on c-MYC G-quadruplex suggest that 9-methoxyluminarine may preferentially interact with 3′end of the c-MYC promoter. The high decrease in 9-MeLM ligand fluorescence upon binding to all tested G4s indicates that 9-methoxyluminarine molecule can be used as a selective fluorescence turn-off probe for parallel G-quadruplexes.

Publisher

MDPI AG

Link

https://www.mdpi.com/1420-3049/29/16/3907/pdf

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