Author:
Zhang Xiaojun,Xing Mengtao,Ma Yangcheng,Zhang Zhuangli,Qiu Cuipeng,Wang Xiao,Zhao Zhihong,Ji Zhenyu,Zhang Jian-Ying
Abstract
Esophageal squamous cell carcinoma is a severe malignancy for its high mortality and poor prognosis. Mainstay chemotherapies cause serious side effects for their ways of inducing cell death. Oridonin is the main bioactive constituent from natural plants that has anticancer ability and weak side effects. The proteomics method is efficient to understand the anticancer mechanism. However, proteins identified by proteomics aimed at understanding oridonin’s anticancer mechanism is seldom overlapped by different groups. This study used proteomics based on two-dimensional electrophoresis sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2-DE SDS-PAGE) integrated with mass spectrometry and Gene Set Enrichment Analysis (GSEA) to understand the anticancer mechanism of oridonin on esophageal squamous cell carcinoma (ESCC). The results showed that oridonin induced ESCC cell death via apoptosis by decreasing the protein expression of LASP1 and PDLIM1.
Funder
National Institutes on Minority Health and Health Disparities (NIMHD), a component of the National Institutes of Health
a Project of Basic Research Fund of Henan Institute of Medical and Pharmacological Sciences
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference57 articles.
1. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries;Sung;CA Cancer J. Clin.,2021
2. Esophageal cancer;Alsop;Gastroenterol. Clin.,2016
3. Recent advances from basic and clinical studies of esophageal squamous cell carcinoma;Ohashi;Gastroenterology,2015
4. Oesophageal squamous cell carcinoma (ESCC): Advances through omics technologies, towards ESCC salivaomics;Hulak;Drug Discov. Ther.,2015
5. Thun, M., Linet, M.S., Cerhan, J.R., Haiman, C.A., and Schottenfeld, D. (2017). Cancer Epidemiology and Prevention, Oxford University Press.
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