Postbiotics Derived from L. paracasei ET-22 Inhibit the Formation of S. mutans Biofilms and Bioactive Substances: An Analysis
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Published:2023-01-27
Issue:3
Volume:28
Page:1236
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ISSN:1420-3049
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Container-title:Molecules
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language:en
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Short-container-title:Molecules
Author:
Zhao Zhi12, Wu Jianmin3ORCID, Sun Zhe1, Fan Jinbo2, Liu Fudong45, Zhao Wen45, Liu Wei-Hsien45, Zhang Ming1ORCID, Hung Wei-Lian45
Affiliation:
1. School of Food and Health, Beijing Technology and Business University, Beijing 100024, China 2. College of Food Science and Engineering, Bohai University, Jinzhou 121013, China 3. China Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100083, China 4. Inner Mongolia Dairy Technology Research Institute Co., Ltd., Hohhot 010110, China 5. Inner Mongolia Yili Industrial Group Co., Ltd., Hohhot 010110, China
Abstract
Globally, dental caries is one of the most common non-communicable diseases for patients of all ages; Streptococcus mutans (S. mutans) is its principal pathogen. Lactobacillus paracasei (L. paracasei) shows excellent anti-pathogens and immune-regulation functions in the host. The aim of this study is to evaluate the effects of L. paracasei ET-22 on the formation of S. mutans biofilms. The living bacteria, heat-killed bacteria, and secretions of L. paracasei ET-22 were prepared using the same number of bacteria. In vitro, they were added into artificial-saliva medium, and used to coculture with the S. mutans. Results showed that the living bacteria and secretions of L. paracasei ET-22 inhibited biofilm-growth, the synthesis of water-soluble polysaccharide and water-insoluble polysaccharide, and virulence-gene-expression levels related to the formation of S. mutans biofilms. Surprisingly, the heat-killed L. paracasei ET-22, which is a postbiotic, also showed a similar regulation function. Non-targeted metabonomics technology was used to identify multiple potential active-substances in the postbiotics of L. paracasei ET-22 that inhibit the formation of S. mutans biofilms, including phenyllactic acid, zidovudine monophosphate, and citrulline. In conclusion, live bacteria and its postbiotics of L. paracasei ET-22 all have inhibitory effects on the formation of S. mutans biofilm. The postbiotics of L. paracasei ET-22 may be a promising biological anticariogenic-agent.
Funder
National Natural Science Foundation of China China Postdoctoral Science Foundation
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference56 articles.
1. GBD 2017 Disease and Injury Incidence and Prevalence Collaborators (2018). Disease and Injury Incidence and Prevalence Collaborators. Global, regional, and national incidence, prevalence, and years lived with disability for 354 diseases and injuries for 195 countries and territories, 1990–2017: A systematic analysis for the global burden of disease study 2017. Lancet, 392, 1789–1858. 2. Strategies for Streptococcus mutans biofilm dispersal through extracellular polymeric substances disruption;Lin;Mol. Oral Microbiol.,2022 3. Li, J., Wu, T., Peng, W., and Zhu, Y. (2020). Effects of resveratrol on cariogenic virulence properties of Streptococcus mutans. BMC Microbiol., 20. 4. The biofilm matrix;Flemming;Nat. Rev. Microbiol.,2010 5. Bidossi, A., De Grandi, R., Toscano, M., Bottagisio, M., De Vecchi, E., Gelardi, M., and Drago, L. (2018). Probiotics Streptococcus salivarius 24SMB and Streptococcus oralis 89a interfere with biofilm formation of pathogens of the upper respiratory tract. BMC Infect. Dis., 18.
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