Dragon’s Blood Sap Microencapsulation within Whey Protein Concentrate and Zein Using Electrospraying Assisted by Pressurized Gas Technology
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Published:2023-05-17
Issue:10
Volume:28
Page:4137
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ISSN:1420-3049
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Container-title:Molecules
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language:en
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Short-container-title:Molecules
Author:
Escobar-García Juan David1ORCID, Prieto Cristina2ORCID, Pardo-Figuerez Maria12ORCID, Lagaron Jose M.2ORCID
Affiliation:
1. Research & Development Department, Bioinicia S.L. Calle Algepser 65, 46980 Paterna, Spain 2. Novel Materials and Nanotechnology Group, Institute of Agrochemistry and Food Technology (IATA), Spanish Council for Scientific Research (CSIC), Calle Catedrático Agustín Escardino Benlloch 7, 46980 Paterna, Spain
Abstract
Dragon’s blood sap (DBS) obtained from the bark of Croton lechleri (Müll, Arg.) is a complex herbal remedy of pharmacological interest due to its high content in polyphenols, specifically proanthocyanidins. In this paper, electrospraying assisted by pressurized gas (EAPG) was first compared with freeze-drying to dry natural DBS. Secondly, EAPG was used for the first time to entrap natural DBS at room temperature into two different encapsulation matrices, i.e., whey protein concentrate (WPC) and zein (ZN), using different ratios of encapsulant material: bioactive compound, for instance 2:1 w/w and 1:1 w/w. The obtained particles were characterized in terms of morphology, total soluble polyphenolic content (TSP), antioxidant activity, and photo-oxidation stability during the 40 days of the experiment. Regarding the drying process, EAPG produced spherical particles with sizes of 11.38 ± 4.34 µm, whereas freeze-drying produced irregular particles with a broad particle size distribution. However, no significant differences were detected between DBS dried by EAPG or freeze-drying in TSP, antioxidant activity, and photo-oxidation stability, confirming that EAPG is a mild drying process suitable to dry sensitive bioactive compounds. Regarding the encapsulation process, the DBS encapsulated within the WPC produced smooth spherical microparticles, with average sizes of 11.28 ± 4.28 µm and 12.77 ± 4.54 µm for ratios 1:1 w/w and 2:1 w/w, respectively. The DBS was also encapsulated into ZN producing rough spherical microparticles, with average sizes of 6.37 ± 1.67 µm and 7.58 ± 2.54 µm for ratios 1:1 w/w and 2:1 w/w, respectively. The TSP was not affected during the encapsulation process. However, a slight reduction in antioxidant activity measured by DPPH was observed during encapsulation. An accelerated photo-oxidation test under ultraviolet light confirmed that the encapsulated DBS showed an increased oxidative stability in comparison with the non-encapsulated DBS, with the stability being enhanced for the ratio of 2:1 w/w. Among the encapsulating materials and according to the ATR-FTIR results, ZN showed increased protection against UV light. The obtained results demonstrate the potential of EAPG technology in the drying or encapsulation of sensitive natural bioactive compounds in a continuous process available at an industrial scale, which could be an alternative to freeze-drying.
Subject
Chemistry (miscellaneous),Analytical Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Molecular Medicine,Drug Discovery,Pharmaceutical Science
Reference50 articles.
1. Kesavan, P., Banerjee, A.A., Banerjee, A.A., Murugesan, R., Marotta, F., and Pathak, S. (2018). An Overview of Dietary Polyphenols and Their Therapeutic Effects, Elsevier Inc.. [2nd ed.]. 2. Achmadi, S.S., Aguilar, C.N., Aguirre, A., Amenta, M., Andabaka, Ž., Aramouni, F., Ašperger, D., Ballistreri, G., Barros, A.I.R.N.A., and Borneo, R. (2019). Polyphenols in Plants, Elsevier Inc. 3. Niaz, K., and Khan, F. (2020). Chapter 3—Analysis of Polyphenolics. Recent Adv. Nat. Prod. Anal., 39–197. 4. Belščak-Cvitanović, A., Durgo, K., Huđek, A., Bačun-Družina, V., and Komes, D. (2018). Overview of Polyphenols and Their Properties, Elsevier. 5. Thakur, M., Singh, K., and Khedkar, R. (2020). Functional and Preservative Properties of Phytochemicals, Elsevier.
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