Affiliation:
1. Institute of Physical Chemistry ‘Acad. R. Kaischew’, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
Abstract
The kinetics of amyloid aggregation was studied indirectly by monitoring the changes in the polydispersity of mixed dispersion of amyloid β peptide (1-40) and composite liposomes. The liposomes were prepared from the 1,2-dioleoyl-sn-glicero-3-phoshocholine (DOPC) phospholipid and stabilised by the electrostatic adsorption of κ-carrageenan. The produced homotaurine-loaded and unloaded liposomes had a highly negative electrokinetic potential and remarkable stability in phosphate buffer (pH 4 and 7.4). For the first time, the appearance and evolution of the aggregation of Aβ were presented through the variation in the standard percentile readings (D10, D50, and D90) obtained from the particle size distribution analysis. The kinetic experiments indicated the appearance of the first aggregates almost 30 min after mixing the liposomes and peptide solution. It was observed that by adding unloaded liposomes, the size of 90% of the particles in the dispersion (D90) increased. In contrast, the addition of homotaurine-loaded liposomes had almost minimal impact on the size of the fractions of larger particles during the kinetic experiments. Despite the specific bioactivity of homotaurine in the presence of natural cell membranes, this study reported an additional inhibitory effect of the compound on the amyloid peptide aggregation due to the charge effects and ‘molecular crowding’.
Reference31 articles.
1. (2023, December 08). World Alzheimer Report 2023. Available online: www.alzint.org/resource/world-alzheimer-report-2023/.
2. Russo, O., Kisialiou, A., Lamonaca, P., Moroni, R., Prinzi, G., and Fimi, M. (2016). New drugs from marine organisms in Alzheimer’s disease. Mar. Drugs, 14.
3. Aggregation and structure of amyloid β-protein;Ono;Neurochem. J.,2021
4. Alzheimer’s disease as oligomeropathy;Ono;Neurochem. Int.,2018
5. Seeding ‘one-dimensional crystallization’ of amyloid: A pathogenic mechanism in Alzheimer’s disease and scrapie?;Jarrett;Cell,1993