Author:
Ramalingam Raghavendra,Dhand Chetna,Leung Chak,Ezhilarasu Hariharan,Prasannan Praseetha,Ong Seow,Subramanian Sundarapandian,Kamruddin Mohammed,Lakshminarayanan Rajamani,Ramakrishna Seeram,Verma Navin,Arunachalam Kantha
Abstract
Electrospun fibers have emerged as promising materials in the field of biomedicine, due to their superior physical and cell supportive properties. In particular, electrospun mats are being developed for advanced wound dressing applications. Such applications require the firers to possess excellent antimicrobial properties in order to inhibit potential microbial colonization from resident and non-resident bacteria. In this study, we have developed Poly-ε-Caprolactone /gelatin hybrid composite mats loaded with natural herbal extract (Gymnema sylvestre) to prevent bacterial colonization. As-spun scaffolds exhibited good wettability and desirable mechanical properties retaining their fibrous structure after immersing them in phosphate buffered saline (pH 7.2) for up to 30 days. The initial burst release of Gymnema sylvestre prevented the colonization of bacteria as confirmed by the radial disc diffusion assay. Furthermore, the electrospun mats promoted cellular attachment, spreading and proliferation of human primary dermal fibroblasts and cultured keratinocytes, which are crucial parenchymal cell-types involved in the skin recovery process. Overall these results demonstrated the utility of Gymnema sylvestre impregnated electrospun PCL/Gelatin nanofibrous mats as an effective antimicrobial wound dressing.
Funder
National Research Foundation Singapore
Singapore National Medical Research Council
Subject
General Materials Science,General Chemical Engineering
Cited by
59 articles.
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