Molecular Surveillance of Artemisinin-Resistant Plasmodium falciparum Parasites in Mining Areas of the Roraima Indigenous Territory in Brazil

Author:

de Aguiar-Barros Jacqueline12ORCID,Granja Fabiana234,de Abreu-Fernandes Rebecca56,de Queiroz Lucas Tavares56,da Silva e Silva Daniel3,Citó Arthur Camurça7ORCID,Mocelin Natália Ketrin Almeida-de-Oliveira56,Daniel-Ribeiro Cláudio Tadeu56,Ferreira-da-Cruz Maria de Fátima56ORCID

Affiliation:

1. Malaria Control Center, Epidemiological Surveillance Department, General Health Surveillance Coordination, SESAU-RR, Roraima 69305-080, Brazil

2. Postgraduate Program in Biodiversity and Biotechnology–BIONORTE Network/Roraima Federal University (UFRR), Roraima 69310-000, Brazil

3. Biodiversity Research Centre, Roraima Federal University (UFRR), Roraima 69304-000, Brazil

4. Graduate Program in Natural Resources, Federal University of Roraima (UFRR), Roraima 69304-000, Brazil

5. Laboratório de Pesquisa em Malária, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-360, Brazil

6. Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal)/Reference Center for Malaria in the Extra-Amazon Region of the Brazilian Ministry of Health, Fiocruz, Rio de Janeiro 21040-900, Brazil

7. Research Support Center in Roraima (NAPRR) of the National Institute for Amazonian Research (INPA), Roraima 69301-150, Brazil

Abstract

Multidrug- and artemisinin-resistant (ART-R) Plasmodium falciparum (Pf) parasites represent a challenge for malaria elimination worldwide. Molecular monitoring in the Kelch domain region (pfk13) gene allows tracking mutations in parasite resistance to artemisinin. The increase in illegal miners in the Roraima Yanomami indigenous land (YIL) could favor ART-R parasites. Thus, this study aimed to investigate ART-R in patients from illegal gold mining areas in the YIL of Roraima, Brazil. A questionnaire was conducted, and blood was collected from 48 patients diagnosed with P. falciparum or mixed malaria (Pf + P. vivax). The DNA was extracted and the pfk13 gene was amplified by PCR. The amplicons were subjected to DNA-Sanger-sequencing and the entire amplified fragment was analyzed. Among the patients, 96% (46) were from illegal mining areas of the YIL. All parasite samples carried the wild-type genotypes/ART-sensitive phenotypes. These data reinforce the continued use of artemisinin-based combination therapies (ACTs) in Roraima, as well as the maintenance of systematic monitoring for early detection of parasite populations resistant to ART, mainly in regions with an intense flow of individuals from mining areas, such as the YIL. This is especially true when the achievement of falciparum malaria elimination in Brazil is planned and expected by 2030.

Funder

Department of Science and Technology in Health/Ministry of Health

the Oswaldo Cruz Foundation

the Carlos Chagas Filho Foundation for Research Support in the State of Rio de Janeiro

Health Surveillance Secretariat/Ministry of Health

Federal University of Roraima

MFFC and CTDR are supported by CNPq, Brazil, through Research Productivity Fellowships

Publisher

MDPI AG

Reference55 articles.

1. World Health Organization (2023). World Malaria Report 2022, World Health Organization.

2. Brazil (2024, April 13). Ministry of Health. Available online: https://public.tableau.com/app/profile/mal.ria.brasil/viz/Dadosparacidado_201925_03_2020/Incio.

3. Brazil Ministry of Health (2022). Epidemiological Bulletin—Malaria 2021, Ministry of Health.

4. World Health Organization (2015). Global Technical Strategy For Malaria 2016–2030, World Health Organization.

5. (2022). Eliminate Malaria Brazil: National Malaria Elimination Plan, Brazil, Ministry of Health, Health Surveillance Secretariat, Department of Immunization and Communicable Diseases.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3