The Role of Globularia alypum Explored Ex Vivo In Vitro on Human Colon Biopsies from Ulcerative Colitis Patients

Author:

Hajji Najla1ORCID,Russo Ilaria1ORCID,Bianco Jessica1ORCID,Piazza Ornella1ORCID,Iovino Paola1ORCID,Santonicola Antonella1ORCID,Ciacci Carolina1ORCID

Affiliation:

1. Department of Medicine, Surgery and Dentistry, Scuola Medica Salernitana, University of Salerno, Baronissi, 84081 Salerno, Italy

Abstract

The existing literature indicates that Globularia alypum L. (GA) influences inflammation and oxidative stress modulation in rats and in vitro. The present study aims to investigate the effects of this plant in patients with ulcerative colitis (UC) and normal controls. In our experiments, we pretreated colon biopsies from 46 UC patients and normal controls with GA leaves aqueous extract (GAAE) used at two concentrations (50 and 100 µg/mL) for 3 h, followed by Lipopolysaccharides (from Escherichia coli) stimulation. We analyzed the effects on inflammation by studying the cyclo-oxygenase-2, the intercellular adhesion molecule-1, the nuclear factor kappa B, and p38 mitogen-activated protein kinase expression. Moreover, we assessed the levels of interleukin 6, the superoxide dismutase activity, and nitric oxide release in the supernatant of cultures. Our data showed that GAAE influences UC patients and normal controls for most studied markers and enzymes. These results acknowledge, with some scientific evidence, the traditional belief in the anti-inflammatory properties of GA and represent the first demonstration of its effect in a human in vitro model of inflammatory conditions.

Publisher

MDPI AG

Subject

Food Science,Nutrition and Dietetics

Reference38 articles.

1. Globularia alypum methanolic extract improves burn wound healing process and inflammation in rats and possesses antibacterial and antioxidant activities;Ghlissi;Biomed. Pharm.,2016

2. Not Available;Leclerc;La Presse Med.,1948

3. A potential antileukemic substance present in Globularia alypum;Caldes;Planta Med.,1975

4. Phytotherapy of hypertension and diabetes in oriental Morocco;Ziyyat;J. Ethnopharmacol.,1997

5. Molecular Docking Studies, Chemical Composition, Antioxidant, Cytotoxicity, Antibacterial and Antifungal Activities of Globularia alypum Extract;Najla;Curr. Bioact. Compd.,2021

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