Abstract
Ebola virus (EBOV) is a negative-sense RNA virus that can infect humans and nonhuman primates with severe health consequences. Development of countermeasures requires a thorough understanding of the interaction between host and pathogen, and the course of disease. The goal of this study was to further characterize EBOV disease in a uniformly lethal rhesus macaque model, in order to support development of a well-characterized model following rigorous quality standards. Rhesus macaques were intramuscularly exposed to EBOV and one group was euthanized at predetermined time points to characterize progression of disease. A second group was not scheduled for euthanasia in order to analyze survival, changes in physiology, clinical pathology, terminal pathology, and telemetry kinetics. On day 3, sporadic viremia was observed and pathological evidence was noted in lymph nodes. By day 5, viremia was detected in all EBOV exposed animals and pathological evidence was noted in the liver, spleen, and gastrointestinal tissues. These data support the notion that EBOV infection in rhesus macaques is a rapid systemic disease similar to infection in humans, under a compressed time scale. Biomarkers that correlated with disease progression at the earliest stages of infection were observed thereby identifying potential “trigger-to-treat” for use in therapeutic studies.
Funder
Biomedical Advanced Research and Development Authority
National Center for Research Resources
Office of Research Infrastructure Programs, National Institutes of Health
Subject
Virology,Microbiology (medical),Microbiology
Reference43 articles.
1. Filoviruses. A compendium of 40 years of epidemiological, clinical, and laboratory studies;Kuhn;Arch. Virol. Suppl.,2008
2. Emergence of Zaire Ebola Virus Disease in Guinea
3. Ebola and Marburg Viruses;Klenk,2004
4. Pathogenesis of filoviral haemorrhagic fevers
5. Kinetics of Soluble Mediators of the Host Response in Ebola Virus Disease
Cited by
10 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献