New Insights into Microbial Diversity of the Traditional Packed Table Olives Aloreña de Málaga through Metataxonomic Analysis

Author:

López-García Elio,Benítez-Cabello Antonio,Ramiro-García JavierORCID,Romero-Gil Verónica,Rodríguez-Gómez FranciscoORCID,Arroyo-López Francisco NoéORCID

Abstract

Aloreña de Málaga is a table olive especially characterised by its natural freshness and short shelf-life. In this work, we applied a metataxonomic approach to unravel the microbial diversity of bacterial and fungi populations through the shelf-life of traditionally packed Aloreña de Málaga. A significant increase in lactic acid bacteria and mesophilic aerobic populations was observed during shelf-life, reaching the maximum population levels (4–5 log10 CFU) at the end of the study (260 days). On the contrary, a rapid reduction in yeast and mould populations was reported. The use of a metataxonomic analysis based on the amplification of 16S (bacteria) and internal transcribed spacer (ITS) region (fungi) regions revealed a low diversity for both microbial groups. Lactiplantibacillus (65.05 ± 8.65% in brine vs. 58.70 ± 15.70% in fruit), Pediococcus (28.17 ± 7.36% in brine vs. 27.20 ± 15.95% in fruit), and Celerinatantimonas (4.64 ± 1.08% in brine vs. 11.82 ± 18.17% in fruit) were the main genera found among bacteria, and an increase in Lactiplantibacillus and a reduction in Celerinatantimonas populations during the shelf-life were observed. On the other hand, Citeromyces was the dominant fungi genus (54.11 ± 2.00% in brine vs. 50.91 ± 16.14% in fruit), followed by Candida (8.80 ± 2.57% in brine vs. 12.32 ± 8.61% in fruit) and Penicillium (6.48 ± 1.87% vs. 8.48 ± 4.43% in fruit). No food-borne pathogen genera were detected in any of the samples analysed, indicating the high level of food safety found in this ready-to-eat fermented vegetable. Data obtained in this work will help in the design of new strategies for the control of microbial populations during the shelf-life of Aloreña de Málaga.

Funder

Ministerio de Economía, Industria y Competitividad, Gobierno de España

Publisher

MDPI AG

Subject

Virology,Microbiology (medical),Microbiology

Reference45 articles.

1. International Olive Oil Council Economic Affairs & Promotion Unit-International Olive Councilhttps://www.internationaloliveoil.org/what-we-do/economic-affairs-promotion-unit/#figures

2. Table Olives: Production and Processing;Garrido-Fernández,1997

3. Regulation Nº1068/2012. L318/3-L318/4https://www.boe.es/buscar/doc.php?id=DOUE-L-2012-82202

4. Metataxonomic analysis of the bacterial diversity in table olive dressing components

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