Effects of the Inclusion of Fermented Mulberry Leaves and Branches in the Gestational Diet on the Performance and Gut Microbiota of Sows and Their Offspring

Abstract

Fermented feed mulberry (FFM), being rich in dietary fiber, has not been fully evaluated to be used in sow’s diet. In this study, we investigated the effects of 25.5% FFM supplemented in gestation diets on the performance and gut microbiota of sows and their offspring. Results showed that the serum concentration of glucose, progesterone, and estradiol were not affected by the dietary treatment, while the level of serum insulin and fecal short chain fatty acid were both reduced in FFM group on gestation day 60 (G60, p < 0.05). Additionally, FFM increased both voluntary feed intake and weaning litter weight (p < 0.05), while decreased the losses of both Backfat thickness and bodyweight throughout lactation (p < 0.05). 16S rRNA sequencing showed FFM supplementation significantly increased the diversity and relative abundance of sows’ fecal microbiota on G60 (p < 0.05). The differential microbiota for sows from FFM group was that Bacteroidetes was increased on G60 while Firmicutes were decreased on Lactation day 7 (L7, p < 0.05), and which for the FFM piglets was that both unclassified_f_Lachnospiraceae on L0 and norank_f_Ruminococcaceae on L7 were increased (p < 0.05). In short, FFM can be recognized as a potential feed ingredient used in sow’s diet.