Impact of the Deletion of Genes of the Nitrogen Metabolism on Triacylglycerol, Cardiolipin and Actinorhodin Biosynthesis in Streptomyces coelicolor
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Published:2024-07-30
Issue:8
Volume:12
Page:1560
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ISSN:2076-2607
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Container-title:Microorganisms
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language:en
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Short-container-title:Microorganisms
Author:
Abreu Sonia1ORCID, Lejeune Clara2ORCID, David Michelle2, Chaminade Pierre1, Virolle Marie-Joelle2
Affiliation:
1. Lip (Sys)2 (Lipides Systèmes Analytiques et Biologiques), UFR Pharmacie-Bâtiment Henri Moissan, CNRS, CEA, Université Paris-Saclay, 17 Avenue des Sciences, 91400 Orsay, France 2. Institute for Integrative Biology of the Cell (I2BC), Department of Microbiology, Group “Energetic Metabolism of Streptomyces”, CNRS, CEA, Université Paris-Saclay, 1 Avenue de la Terrasse, 91198 Gif-Sur-Yvette, France
Abstract
Since nitrogen limitation is known to be an important trigger of triacylglycerol (TAG) accumulation in most microorganisms, we first assessed the global lipid content of 21 strains derived from Streptomyces coelicolor M145 deleted for genes involved in nitrogen metabolism. Seven of these strains deleted for genes encoding proteins involved in polyamine (GlnA2/SCO2241, GlnA3/SCO6962, GlnA4/SCO1613), or protein (Pup/SCO1646) degradation, in the regulation of nitrogen metabolism (GlnE/SCO2234 and GlnK/SCO5584), or the global regulator DasR/SCO5231 that controls negatively the degradation of N-acetylglucosamine, a constituent of peptidoglycan, had a higher TAG content than the original strain, whereas five of these strains (except the glnA2 and pup mutants) had a lower cardiolipin (CL) content. The production of the blue polyketide actinorhodin (ACT) was totally abolished in the dasR mutant in both Pi conditions, whereas the deletion of pup, glnA2, glnA3, and glnA4 was correlated with a significant increase in total ACT production, but mainly in Pi limitation. Unexpectedly, ACT production was strongly reduced in the glnA3 mutant in Pi proficiency. Altogether, our data suggest that high TAG and ACT biosynthesis and low CL biosynthesis might all contribute to the lowering of oxidative stress resulting from nitrogen limitation or from other causes.
Funder
CNRS University Paris-Saclay ANR BioSound-IR “Direction Générale des Armées” “l’Agence de l’Innovationde Défense”
Reference73 articles.
1. The Absence of Pupylation (Prokaryotic Ubiquitin-Like Protein Modification) Affects Morphological and Physiological Differentiation in Streptomyces coelicolor;Boubakri;J. Bacteriol.,2015 2. Genetic and Proteomic Analyses of Pupylation in Streptomyces coelicolor;Compton;J. Bacteriol.,2015 3. Krysenko, S., Okoniewski, N., Nentwich, M., Matthews, A., Bauerle, M., Zinser, A., Busche, T., Kulik, A., Gursch, S., and Kemeny, A. (2022). A Second Gamma-Glutamylpolyamine Synthetase, GlnA2, Is Involved in Polyamine Catabolism in Streptomyces coelicolor. Int. J. Mol. Sci., 23. 4. Krysenko, S., Okoniewski, N., Kulik, A., Matthews, A., Grimpo, J., Wohlleben, W., and Bera, A. (2017). Gamma-Glutamylpolyamine Synthetase GlnA3 Is Involved in the First Step of Polyamine Degradation Pathway in Streptomyces coelicolor M145. Front. Microbiol., 8. 5. Initial Metabolic Step of a Novel Ethanolamine Utilization Pathway and Its Regulation in Streptomyces coelicolor M145;Krysenko;Mbio,2019
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