Driving Factors Influencing Soil Microbial Community Succession of Coal Mining Subsidence Areas during Natural Recovery in Inner Mongolia Grasslands

Abstract

Soil microorganisms significantly influence the energy flow and material cycle of soil ecosystems, making them highly susceptible to environmental changes, such as those induced by mining activities. Studying the succession of soil microbial communities after mining subsidence is crucial for comprehending the significance of soil microbes in the natural recovery process following subsidence. Therefore, the soil properties, vegetation communities, and soil microbial communities of the subsidence area, as well as unexploited areas, were analyzed during the natural restoration process (1, 2, 5, 10, and 15 years). The results demonstrate that mining subsidence has a significant impact on the aboveground vegetation community, soil properties, and microbiological community. Following an extended period of natural recovery, a new stable state has emerged, which differs from that observed in non-subsidence areas. The total nitrogen, nitrate nitrogen, and ammonium nitrogen amounts may be key factors driving the natural recovery of bacterial communities, and total potassium and available potassium may be key factors driving the natural recovery of fungal communities. The natural recovery mechanism of soil microorganisms was analyzed along with the changes related to vegetation and soil physicochemical properties. The mechanism was explained from three perspectives, namely, plant-led, soil-led, and soil-microbial-led, which could provide a theoretical basis for the natural restoration of grassland ecosystems and provide guidance for the treatment of coal mining subsidence areas.