Advancing Bacillus licheniformis as a Superior Expression Platform through Promoter Engineering
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Published:2024-08-16
Issue:8
Volume:12
Page:1693
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ISSN:2076-2607
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Container-title:Microorganisms
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language:en
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Short-container-title:Microorganisms
Author:
Xiao Fengxu1234, Zhang Yupeng1234, Zhang Lihuan123, Li Siyu123, Chen Wei4, Shi Guiyang123, Li Youran123
Affiliation:
1. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China 2. National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China 3. Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan University, Wuxi 214122, China 4. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Abstract
Bacillus licheniformis is recognised as an exceptional expression platform in biomanufacturing due to its ability to produce high-value products. Consequently, metabolic engineering of B. licheniformis is increasingly pursued to enhance its utility as a biomanufacturing vehicle. Effective B. licheniformis cell factories require promoters that enable regulated expression of target genes. This review discusses recent advancements in the characterisation, synthesis, and engineering of B. licheniformis promoters. We highlight the application of constitutive promoters, quorum sensing promoters, and inducible promoters in protein and chemical synthesis. Additionally, we summarise efforts to expand the promoter toolbox through hybrid promoter engineering, transcription factor-based inducible promoter engineering, and ribosome binding site (RBS) engineering.
Funder
National Key Research & Development Program of China the National Natural Foundation of China the National First-Class Discipline Program of Light Industry Technology and Engineering Top-notch Academic Programs Project of Jiangsu Higher Education Institutions Jiangsu Funding Program for Excellent Postdoctoral Talent
Reference94 articles.
1. Biotechnological applications of Bacillus licheniformis;Muras;Crit. Rev. Biotechnol.,2021 2. Alrumman, S.A., Mostafa, Y.S., Al-Izran, K.A., Alfaifi, M.Y., Taha, T.H., and Elbehairi, S.E. (2019). Production and anticancer activity of an L-Asparaginase from Bacillus licheniformis isolated from the red sea, saudi arabia. Sci. Rep., 9. 3. Prevalence of feather-degrading Bacillus spp. on the plumage of birds in Australia;Sotnychuk;Emu Austral Ornithol.,2019 4. Othoum, G., Bougouffa, S., Razali, R., Bokhari, A., Alamoudi, S., Antunes, A., Gao, X., Hoehndorf, R., Arold, S.T., and Gojobori, T. (2018). In silico exploration of red sea Bacillus genomes for natural product biosynthetic gene clusters. BMC Genom., 19. 5. Liu, J., Zhang, J., Zhu, M., Wan, H., Chen, Z., Yang, N., Duan, J., Wei, Z., Hu, T., and Liu, F. (2022). Effects of plant growth promoting rhizobacteria (PGPR) strain Bacillus licheniformis with biochar amendment on potato growth and water use efficiency under reduced irrigation regime. Agronomy, 12.
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