Application of Fourier Transform Infrared Spectroscopy to Discriminate Two Closely Related Bacterial Species: Bacillus anthracis and Bacillus cereus Sensu Stricto

Author:

Manzulli Viviana1ORCID,Cordovana Miriam2,Serrecchia Luigina1,Rondinone Valeria1ORCID,Pace Lorenzo1ORCID,Farina Donatella1,Cipolletta Dora1,Caruso Marta1,Fraccalvieri Rosa1ORCID,Difato Laura Maria1,Tolve Francesco1,Vetritto Valerio1,Galante Domenico1ORCID

Affiliation:

1. Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy

2. Bruker Daltonics GmbH, 28359 Bremen, Germany

Abstract

Fourier transform infrared spectroscopy (FTIRS) is a diagnostic technique historically used in the microbiological field for the characterization of bacterial strains in relation to the specific composition of their lipid, protein, and polysaccharide components. For each bacterial strain, it is possible to obtain a unique absorption spectrum that represents the fingerprint obtained based on the components of the outer cell membrane. In this study, FTIRS was applied for the first time as an experimental diagnostic tool for the discrimination of two pathogenic species belonging to the Bacillus cereus group, Bacillus anthracis and Bacillus cereus sensu stricto; these are two closely related species that are not so easy to differentiate using classical microbiological methods, representing an innovative technology in the field of animal health.

Publisher

MDPI AG

Subject

Virology,Microbiology (medical),Microbiology

Reference26 articles.

1. Introduction: Anthrax history, disease and ecology;Turnbull;Curr. Top. Microbiol. Immunol.,2002

2. World Health Organization (2008). Anthrax in Humans and Animals, World Health Organization. [4th ed.].

3. Pathogenicity, population genetics and dissemination of Bacillus anthracis;Pilo;Infect. Genet. Evol.,2018

4. Anthrax Pathogenesis;Moayeri;Annu. Rev. Microbiol.,2015

5. An overview of anthrax infection including the recently identified form of disease in injection drug users;Hicks;Intensive Care Med.,2012

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