Epidemiology and Genetic Characterization of Leishmania RNA Virus in Leishmania (Viannia) spp. Isolates from Cutaneous Leishmaniasis Endemic Areas in Panama-Reference-Cited by-同舟云学术

Epidemiology and Genetic Characterization of Leishmania RNA Virus in Leishmania (Viannia) spp. Isolates from Cutaneous Leishmaniasis Endemic Areas in Panama

Published:2024-06-27 Issue:7 Volume:12 Page:1317
ISSN:2076-2607
Container-title:Microorganisms
language:en
Short-container-title:Microorganisms

Author:

Bonilla Armando Assair¹,Pineda Vanessa²^ORCID,Calzada José Eduardo²³^ORCID,Saldaña Azael⁴^ORCID,Laurenti Marcia Dalastra⁵^ORCID,Goya Stephanie⁶^ORCID,Abrego Leyda⁷⁸^ORCID,González Kadir²⁹^ORCID

Affiliation:

1. Programa de Maestría en Ciencias Parasitológicas, Facultad de Ciencias Naturales, Exactas y Tecnología, Universidad de Panamá, Panama 3366, Panama

2. Departamento de Investigación en Parasitología, Instituto de Conmemorativo Gorgas de Estudios de la Salud, Panama 0816-02593, Panama

3. Facultad de Medicina Veterinaria, Universidad de Panamá, Panama 3366, Panama

4. Centro de Investigación y Diagnóstico de Enfermedades Parasitarias (CIDEP), Facultad de Medicina, Universidad de Panamá, Panama 3366, Panama

5. Laboratório de Patologia de Moléstias Infecciosas, Faculdade de Medicina, Universidade de São Paulo, São Paulo 05508-270, SP, Brazil

6. Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195, USA

7. Departamento de Investigación en Virología y Biotecnología, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panama 0816-02593, Panama

8. Departamento de Microbiología y Parasitología, Facultad de Ciencias Naturales, Exactas y Tecnología, Universidad de Panamá, Panama 3366, Panama

9. Departamento de Microbiología Humana, Facultad de Medicina, Universidad de Panamá, Panama 3366, Panama

Abstract

Leishmania (Viannia) spp. can harbor a double-stranded RNA virus known as Leishmania RNA virus 1 (LRV-1), whose presence has been reported in nine countries across the Americas and seven Leishmania species. Here, we studied 100 Leishmania (Viannia) isolates from patients with cutaneous leishmaniasis collected from different endemic areas in Panama from 2016 to 2022. We identified L. (V.) panamensis, L. (V.) guyanensis, L. (V.) braziliensis/guyanensis hybrid, and L. (V.) panamensis sp.1. (genetic variant). LRV-1 was detected by RT-PCR in 9% of L. (Viannia) isolates (eight cases in L. (V.) panamensis, and one in L. (V.) guyanensis). Phylogenetic analysis based on sequencing data classified all LRV-1 isolates within genotype A, suggesting that LRV phylogenetic proximity is closely aligned with geographical distribution or to the phylogenetic proximity of the Leishmania host in the case of the L. (V.) panamensis and L. (V.) guyanensis in Panama.

Funder

SENACYT

Sistema Nacional de Investigación (SNI-SENACYT), Panama

National Council for Scientific and Technological Development (CNPq), Brazil

Gorgas Memorial Institute for Health Studies

Publisher

MDPI AG

Link

https://www.mdpi.com/2076-2607/12/7/1317/pdf

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