Evaluating the Safety of Bacillus cereus GW-01 Obtained from Sheep Rumen Chyme

Author:

Xu Bowen12,Huang Xinyi12,Qin Haixiong12,Lei Ying1,Zhao Sijia12,Liu Shan12,Liu Gang12,Zhao Jiayuan12ORCID

Affiliation:

1. Key Laboratory of Land Resources Evaluation and Monitoring in Southwest, Ministry of Education, Sichuan Normal University, Chengdu 610101, China

2. College of Life Science, Sichuan Normal University, Chengdu 610101, China

Abstract

Bacillus cereus is responsible for 1.4–12% food poisoning outbreaks worldwide. The safety concerns associated with the applications of B. cereus in health and medicine have been controversial due to its dual role as a pathogen for foodborne diseases and a probiotic in humans and animals. In this study, the pathogenicity of B. cereus GW-01 was assessed by comparative genomic, and transcriptome analysis. Phylogenetic analysis based on a single-copy gene showed clustering of the strain GW-01, and 54 B. cereus strains from the NCBI were classified into six major groups (I–VI), which were then associated with the source region and sequence types (STs). Transcriptome results indicated that the expression of most genes related with toxins secretion in GW-01 was downregulated compared to that in the lag phase. Overall, these findings suggest that GW-01 is not directly associated with pathogenic Bacillus cereus and highlight an insightful strategy for assessing the safety of novel B. cereus strains.

Funder

Science and Technology Department of Tibet

National Natural Science Foundation of China

Sichuan Science and Technology Program

Sichuan Normal University

Publisher

MDPI AG

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