Evaluation of the Antifungal and Biochemical Activities of Fungicides and Biological Agents against Ginseng Sclerotinia Root Rot Caused by Sclerotinia nivalis-Reference-Cited by-同舟云学术

Evaluation of the Antifungal and Biochemical Activities of Fungicides and Biological Agents against Ginseng Sclerotinia Root Rot Caused by Sclerotinia nivalis

Published:2024-08-25 Issue:9 Volume:12 Page:1761
ISSN:2076-2607
Container-title:Microorganisms
language:en
Short-container-title:Microorganisms

Author:

Feng Shi¹,Wang Chunlin¹,Xu Zhaoyang¹,Dou Baozhu¹,Wang Xue¹,Yang Lina¹,Lu Baohui¹^ORCID,Gao Jie²

Affiliation:

1. College of Plant Protection, Jilin Agricultural University, Changchun 130118, China

2. State-Local Joint Engineering Research Center of Ginseng Breeding and Application, Changchun 130118, China

Abstract

The objective of this study was to identify effective agents for the prevention and control of ginseng Sclerotinia root rot disease caused by Sclerotinia nivalis. The inhibitory effects of 16 chemical fungicides and 10 biocontrol agents (strains) on mycelial growth and sclerotium formation in S. nivalis were determined using a plate confrontation essay. The results showed that the best chemical agents for inhibiting the mycelial growth and sclerotium formation of S. nivalis were fluconazole and fludioxonil, while Bacillus amyloliquefaciens FS6 and B. subtilis (Kono) were the best biocontrol agents (strains). The results of field trials in 2022 and 2023 showed that the control effects of fluconazole and fludioxonil on ginseng Sclerotinia root rot disease were 90.60–98.16%, and those of the biocontrol agents B. amyloliquefaciens FS6 and B. subtilis (Kono) were 94.80–97.24%, respectively. Chemical agents produced abnormal and twisted mycelia, while the biocontrol agents increased mycelial branching, dilated the mycelium tip, and revealed an abnormal balloon. All of the fungicides decreased the ergosterol content, changed the cell membrane permeability, and increased the protein and nucleic acid permeability. These results suggest that these are potential agents for controlling ginseng Sclerotinia root rot disease, and their biochemical mechanisms of chemical and biocontrol of this disease were demonstrated.

Funder

National Natural Science Foundation of China

Project of Science and Technology Development Plan of Jilin Province

Overseas Expertise Introduction Project for Discipline Innovation

earmarked fund for China Agriculture Research System

Publisher

MDPI AG

Link

https://www.mdpi.com/2076-2607/12/9/1761/pdf

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