Intestinal Effects of Filtered Alkalinized Water in Lean and Obese Zucker Rats
-
Published:2024-02-02
Issue:2
Volume:12
Page:316
-
ISSN:2076-2607
-
Container-title:Microorganisms
-
language:en
-
Short-container-title:Microorganisms
Author:
Doblado Laura1ORCID, Díaz Ligia Esperanza2ORCID, Nova Esther2ORCID, Marcos Ascensión2, Monsalve María1ORCID
Affiliation:
1. Instituto de Investigaciones Biomédicas Sols-Morreale (CSIC-UAM), Arturo Duperier 4, 28029 Madrid, Spain 2. Institute of Science, Food Technology and Nutrition (ICTAN), Spanish National Research Council (CSIC), José Antonio Nováis 6, 28040 Madrid, Spain
Abstract
This study evaluated the intestinal effects of alkalinized filtered water in lean and obese adult Zucker rats. For 3 months, 12-week-old rats consumed either tap water or filtered alkalinized tap water from Madrid city. Weight gain was monitored, changes in metabolism were evaluated by indirect calorimetry, and total antioxidant capacity and levels of inflammatory mediators were measured in plasma. Feces were collected, their microbial composition was analyzed and histological analysis of the small and large intestine was performed, assessing the general state of the mucosa (MUC2), the inflammatory state (F4/80) and the presence of oxidative modifications in protein 4-Hydroxynonenal (4-HNE) by immunofluorescence (IF) and immunohistochemistry (IHC). The results obtained showed that the consumption of alkalinized filtered water improved the composition of the intestinal microbiome and the state of the intestinal mucosa, reducing both local and systemic inflammation and the level of oxidative stress. These changes were accompanied by a better maintenance of the oxidative status in rats. No differences were observed in antioxidant capacity nor in weight gain. The incorporation of probiotics in the diet had a significant impact on the microbiome. These effects were indicative of an improvement in general metabolic, oxidative and inflammatory status.
Funder
Alkanatur under the supporting technological contract STOMAC-ALK MCIN/AEI
Subject
Virology,Microbiology (medical),Microbiology
Reference35 articles.
1. Global, Regional, and National Burden of 10 Digestive Diseases in 204 Countries and Territories from 1990 to 2019;Wang;Front. Public Health,2023 2. Epidemiology, Clinical Characteristics, and Associations for Symptom-Based Rome IV Functional Dyspepsia in Adults in the USA, Canada, and the UK: A Cross-Sectional Population-Based Study;Aziz;Lancet Gastroenterol. Hepatol.,2018 3. Management of Functional Dyspepsia: State of the Art and Emerging Therapies;Yamawaki;Ther. Adv. Chronic Dis.,2018 4. Adverse Effects Associated with Proton Pump Inhibitor Use;Yibirin;Cureus,2021 5. Bajgai, J., Lee, M., Jang, Y.-G., Lee, K., Sharma, S., Jeong, Y.J., Park, H.J., Goh, S.H., Kim, C.-S., and Kim, H.I. (2023). Effects of Drinking Electrolyzed Alkaline-Reduced Water on Functional Dyspepsia: A Randomized, Double-Blind, Controlled Prospective Trial. Processes, 11.
|
|