Genomic Analysis of a Novel Torradovirus “Rehmannia Torradovirus Virus”: Two Distinct Variants Infecting Rehmannia glutinosa

Author:

Qin Yanhong1ORCID,Lu Shuhao1ORCID,Wen Yi1ORCID,Li Shaojian1ORCID,Gao Suxia1,Zhang Desheng1,Li Xuemeng1,Yang Jin1,Gu Li2ORCID,Li Mingjie2,Wang Fei1,Lu Chuantao1

Affiliation:

1. Institute of Plant Protection, Henan Academy of Agricultural Sciences, No. 116, Huanyuan Road, Jinshui District, Zhengzhou 450002, China

2. College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Abstract

Rehmannia glutinosa, a crucial medicinal plant native to China, is extensively cultivated across East Asia. We used high-throughput sequencing to identify viruses infecting R. glutinosa with mosaic, leaf yellowing, and necrotic symptoms. A novel Torradovirus, which we tentatively named “Rehmannia torradovirus virus” (ReTV), was identified. The complete sequences were obtained through reverse-transcription polymerase chain reaction (RT-PCR), 5′ and 3′ rapid amplification of cDNA ends, and Sanger sequencing. The amino acid sequence alignment between the ReTV-52 isolate and known Torradovirus species in the Pro-Pol and coat protein regions were 51.3–73.3% and 37.1–68.1%, respectively. Meanwhile, the amino acid sequence alignment between the ReTV-8 isolate and known Torradovirus species in the Pro-Pol and coat protein regions were 52.7–72.8% and 36.8–67.5%, respectively. The sequence analysis classified ten ReTV strains into two variants. The ReTV-52 genome has two RNA segments of 6939 and 4569 nucleotides, while that of ReTV-8 consists of two RNA segments containing 6889 and 4662 nucleotides. Sequence comparisons and phylogenetic analysis showed ReTV strains clustered within the Torradovirus, exhibiting the closet relation to the squash chlorotic leaf spot virus. The RT-PCR results showed a 100% ReTV detection rate in all 60 R. glutinosa samples. Therefore, ReTV should be classified as a novel Torradovirus species. ReTV is potentially dangerous to R. glutinosa, and necessitating monitoring this virus in the field.

Funder

China Agriculture Research System of MOF and MARA

Henan Modern Industrial Technology System of Chinese Herbal Medicine

New Discipline Development Project of Henan Academy of Agricultural Sciences

independent innovation project of Henan Academy of Agricultural Sciences

Publisher

MDPI AG

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