Genome-Wide Association Study Reveals Host Factors Affecting Conjugation in Escherichia coli

Author:

Van Wonterghem LaetitiaORCID,De Chiara Matteo,Liti Gianni,Warringer JonasORCID,Farewell AnneORCID,Verstraeten NatalieORCID,Michiels Jan

Abstract

The emergence and dissemination of antibiotic resistance threaten the treatment of common bacterial infections. Resistance genes are often encoded on conjugative elements, which can be horizontally transferred to diverse bacteria. In order to delay conjugative transfer of resistance genes, more information is needed on the genetic determinants promoting conjugation. Here, we focus on which bacterial host factors in the donor assist transfer of conjugative plasmids. We introduced the broad-host-range plasmid pKJK10 into a diverse collection of 113 Escherichia coli strains and measured by flow cytometry how effectively each strain transfers its plasmid to a fixed E. coli recipient. Differences in conjugation efficiency of up to 2.7 and 3.8 orders of magnitude were observed after mating for 24 h and 48 h, respectively. These differences were linked to the underlying donor strain genetic variants in genome-wide association studies, thereby identifying candidate genes involved in conjugation. We confirmed the role of fliF, fliK, kefB and ucpA in the donor ability of conjugative elements by validating defects in the conjugation efficiency of the corresponding lab strain single-gene deletion mutants. Based on the known cellular functions of these genes, we suggest that the motility and the energy supply, the intracellular pH or salinity of the donor affect the efficiency of plasmid transfer. Overall, this work advances the search for targets for the development of conjugation inhibitors, which can be administered alongside antibiotics to more effectively treat bacterial infections.

Funder

TransPred

Research Foundation Flanders

Publisher

MDPI AG

Subject

Virology,Microbiology (medical),Microbiology

Reference102 articles.

1. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis

2. Antimicrobial Resistance in the EU/EEA (EARS-Net)—Annual Epidemiological Report 2019,2020

3. Pathogenic Escherichia coli

4. 2020 Antibacterial Agents in Clinical and Preclinical Development: An Overview and Analysis,2021

5. Plasmid encoded antibiotic resistance: acquisition and transfer of antibiotic resistance genes in bacteria

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