Identification of Linear Epitopes in the C-Terminal Region of ASFV p72 Protein
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Published:2023-11-23
Issue:12
Volume:11
Page:2846
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ISSN:2076-2607
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Container-title:Microorganisms
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language:en
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Short-container-title:Microorganisms
Author:
Hu Yifan1, Wang Anchen12, Yan Wanwan1, Li Junbo13, Meng Xin13, Chen Lingchao1, Li Songnan1, Tong Wu1, Kong Ning1, Yu Lingxue1, Yu Hai1ORCID, Shan Tongling1, Xu Jiaping2, Tong Guangzhi1, Zheng Hao14ORCID
Affiliation:
1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China 2. College of Life Sciences, Anhui Agricultural University, Hefei 230031, China 3. College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China 4. Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou 225009, China
Abstract
African swine fever, which is induced by the African swine fever virus (ASFV), poses a significant threat to the global pig industry due to its high lethality in domestic pigs and wild boars. Despite the severity of the disease, there is a lack of effective vaccines and drugs against the ASFV. The p72 protein, constituting 31 to 33% of the total virus particle mass, serves as the primary capsid protein of ASFV. It is a crucial antigen for the development of ASF subunit vaccines and serological diagnostic methods. In this investigation, 27 monoclonal antibodies (mAbs) were generated through mouse immunization with the truncated C-terminal p72 protein expressed by Escherichia coli. Among these, six mAbs exhibited binding to the p72 trimer, with their respective recognized epitopes identified as 542VTAHGINLIDKF553, 568GNAIKTP574, and 584FALKPREEY592. All three epitopes were situated within the interval sequences of functional units of the C-terminal jelly-roll barrel of p72. Notably, two epitopes, 568GNAIKTP574 and 584FALKPREEY592, were internal to the p72 trimer, while the epitope 542VTAHGINLIDKF553 was exposed on the surface of the trimer and consistently conserved across all ASFV genotypes. These findings enhance our comprehension of the antigenic function and structure of the p72 protein, facilitating the utilization of p72 in the development of diagnostic techniques for ASFV.
Funder
National Key Research and Development Program of China Shanghai Science and Technology Innovation Action Plan Shanghai Municipal Natural Science Foundation
Subject
Virology,Microbiology (medical),Microbiology
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