Peptidoglycan Endopeptidase from Novel Adaiavirus Bacteriophage Lyses Pseudomonas aeruginosa Strains as Well as Arthrobacter globiformis and A. pascens Bacteria

Author:

Petrzik Karel1ORCID

Affiliation:

1. Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences, Branisovska 1160/31, 370 05 Ceske Budejovice, Czech Republic

Abstract

A novel virus lytic for Pseudomonas aeruginosa has been purified. Its viral particles have a siphoviral morphology with a head 60 nm in diameter and a noncontractile tail 184 nm long. The dsDNA genome consists of 16,449 bp, has cohesive 3′ termini, and encodes 28 putative proteins in a single strain. The peptidoglycan endopeptidase encoded by ORF 16 was found to be the lytic enzyme of this virus. The recombinant, purified enzyme was active up to 55 °C in the pH range 6–9 against all tested isolates of P. aeruginosa, but, surprisingly, also against the distant Gram-positive micrococci Arthrobacter globiformis and A. pascens. Both this virus and its endolysin are further candidates for possible treatment against P. aeruginosa and probably also other bacteria.

Funder

Czech Academy of Sciences

MEYS CR

ERDF

Publisher

MDPI AG

Subject

Virology,Microbiology (medical),Microbiology

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