Affiliation:
1. Department of Biotechnology and Food Technology, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa
Abstract
Fermented foods are thought to provide a source of probiotics that promote gut health. Consequently, isolation and characterization of fermented food strains and their applications in a controlled fermentation process or as probiotics present a new facet in this area of research. Therefore, the current study sought to identify dominant strains in sorghum-fermented foods (ting) and characterize their probiotic potential in vitro. Recovered isolates were identified as Lactobacillus helveticus, Lactobacillus amylolyticus, Lacticaseibacillus paracasei, Lacticaseibacillus paracasei subsp paracasei, Lactiplantibacillus plantarum, Levilactobacillus brevis, Loigolactobacillus coryniformis and Loigolactobacillus coryniformis subsp torquens based on the their 16S rRNA sequences. Increased biomass was noted in seven out of nine under a low pH of 3 and a high bile concentration of 2% in vitro. Bactericidal activities of isolated LABs presented varying degrees of resistance against selected pathogenic bacteria ranging between (1.57 to 41 mm), (10 to 41 mm), and (11.26 to 42 mm) for Salmonella typhimurium ATTC 14028, Staphylococcus aureus ATTC 6538 and Escherichia coli ATTC8739, respectively. Ampicillin, erythromycin, mupirocin, tetracycline and chloramphenicol were able to inhibit growth of all selected LABs. Thus, isolates recovered from ting partially satisfy the potential candidacy for probiotics by virtue of being more tolerant to acid and bile, antibacterial activity and antibiotic resistance.
Funder
NRF
Tshwane University of Technology
Subject
Virology,Microbiology (medical),Microbiology
Reference54 articles.
1. African Fermented Foods and Probiotics;Franz;Int. J. Food Microbiol.,2014
2. Dimidi, E., Cox, S.R., Rossi, M., and Whelan, K. (2019). Fermented Foods: Definitions and Characteristics, Impact on the Gut Microbiota and Effects on Gastrointestinal Health and Disease. Nutrients, 11.
3. Nyanzi, R., and Jooste, P.J. (2012). Cereal-Based Functional Foods. Probiotics, 161–197.
4. Safety and Quality of Bacterially Fermented Functional Foods and Beverages: A Mini Review;Ashaolu;Food Qual. Saf.,2020
5. Nyanzi, R. (2013). Identification and Properties of Potential Probiotic Bacteria for Application in Mageu, Tshwane University of Technology.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献