Molecular Characterization of Salmonella Phage Wara Isolated from River Water in Brazil-Reference-Cited by-同舟云学术

Molecular Characterization of Salmonella Phage Wara Isolated from River Water in Brazil

Published:2023-07-19 Issue:7 Volume:11 Page:1837
ISSN:2076-2607
Container-title:Microorganisms
language:en
Short-container-title:Microorganisms

Author:

Romero-Calle Danitza Xiomara¹²³^ORCID,Pedrosa-Silva Francisnei⁴^ORCID,Ribeiro Tomé Luiz Marcelo²^ORCID,Fonseca Vagner⁵⁶^ORCID,Guimarães Benevides Raquel¹³,de Oliveira Santos Leila Thaise Santana³,de Oliveira Tulio⁷,da Costa Mateus Matiuzzi⁸,Alcantara Luiz Carlos Junior⁶⁹^ORCID,de Carvalho Azevedo Vasco Ariston⁶^ORCID,Brenig Bertram¹⁰^ORCID,Venancio Thiago M.⁴^ORCID,Billington Craig¹¹^ORCID,Góes-Neto Aristóteles¹²³^ORCID

Affiliation:

1. Postgraduate Program in Biotechnology, State University of Feira de Santana (UEFS), Av. Transnordestina S/N, Feira de Santana 44036-900, Brazil

2. Molecular and Computational Biology of Fungi Laboratory, Department of Microbiology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil

3. Department of Biological Sciences, Feira de Santana State University (UEFS), Feira de Santana 44036-900, Brazil

4. Laboratory of Chemistry, Function of Proteins and Peptides, Center for Biosciences and Biotechnology, Darcy Ribeiro North Fluminense State University (UENF), Campos dos Goytacazes 28013-602, Brazil

5. General Coordination of Public Health Laboratories/Secretariat of Health Surveillance, Ministry of Health, Brasília 70800-400, Brazil

6. Laboratory of Cellular and Molecular Genetics, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil

7. KwaZulu-Natal Research Innovation and Sequencing Platform (KRISP), College of Health Sciences, University of KwaZulu Natal, Durban 4001, South Africa

8. Department of Biological Sciences, Federal University of the São Francisco Valley, Petrolina 56304-917, Brazil

9. Flavivirus Laboratory, Oswaldo Cruz Institute, Rio de Janeiro 21040-900, Brazil

10. Institute of Veterinary Medicine, University of Göttingen, 37073 Göttingen, Germany

11. Health & Environment Group, Institute of Environmental Sciences and Research, Christchurch 8540, New Zealand

Abstract

Antimicrobial resistance is increasing despite new treatments being employed, so novel strategies are required to ensure that bacterial infections remain treatable. Bacteriophages (phages; bacteria viruses) have the potential to be used as natural antimicrobial methods to control bacterial pathogens such as Salmonella spp. A Salmonella phage, Wara, was isolated from environmental water samples at the Subaé River Basin, Salvador de Bahia, Brazil. The basin has environmental impacts in its main watercourses arising from the dumping of domestic and industrial effluents and agricultural and anthropological activities. The phage genome sequence was determined by Oxford Nanopore Technologies (ONT) MinION and Illumina HiSeq sequencing, and assembly was carried out by Racon (MinION) and Unicycler (Illumina, Illumina + MinION). The genome was annotated and compared to other Salmonella phages using various bioinformatics approaches. MinION DNA sequencing combined with Racon assembly gave the best complete genome sequence. Phylogenetic analysis revealed that Wara is a member of the Tequintavirus genus. A lack of lysogeny genes, antimicrobial resistance, and virulence genes indicated that Wara has therapeutic and biocontrol potential against Salmonella species in healthcare and agriculture.

Funder

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil

ESR Strategic Science Initiatives Funding

Publisher

MDPI AG

Subject

Virology,Microbiology (medical),Microbiology

Link

https://www.mdpi.com/2076-2607/11/7/1837/pdf

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