The Effect of Bifidobacterium animalis subsp. lactis Bl-04 on Influenza A Virus Infection in Mice
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Published:2023-10-17
Issue:10
Volume:11
Page:2582
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ISSN:2076-2607
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Container-title:Microorganisms
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language:en
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Short-container-title:Microorganisms
Author:
Zabel Bryan1ORCID, Mäkelä Sanna M.2ORCID, Nedveck Derek1, Hibberd Ashley A.1ORCID, Yeung Nicolas2ORCID, Latvala Sinikka2, Lehtoranta Liisa2, Junnila Jouni3ORCID, Walters Kevin B.4ORCID, Morovic Wesley1ORCID, Lehtinen Markus J.2ORCID
Affiliation:
1. Health & Biosciences, International Flavors & Fragrances, 3329 Agriculture Dr., Madison, WI 53716, USA 2. Health & Biosciences, International Flavors & Fragrances, Sokeritehtaantie 20, 02460 Kantvik, Finland 3. 4Pharma Ltd., Tykistökatu 4D, 20520 Turku, Finland 4. Department of Infectious Disease Research, Southern Research Institute, 431 Aviation Way, Frederick, MD 21701, USA
Abstract
Influenza A virus infection is a major global disease requiring annual vaccination. Clinical studies indicate that certain probiotics may support immune function against influenza and other respiratory viruses, but direct molecular evidence is scarce. Here, mice were treated with a placebo or Bifidobacterium animalis subsp. lactis Bl-04 (Bl-04) orally via food (cereal) and also by gavage and exposed to Influenza A virus H1N1 (H1N1). The symptoms of the infection were observed, and tissues and digesta were collected for viral load RT-qPCR, transcriptomics, and microbiomics. The treatment decreased the viral load by 48% at day 3 post-infection in lungs and symptoms of infection at day 4 compared to placebo. Tissue transcriptomics showed differences between the Bl-04 and placebo groups in the genes in the Influenza A pathway in the intestine, blood, and lungs prior to and post-infection, but the results were inconclusive. Moreover, 16S rRNA gene profiling and qPCR showed the presence of Bl-04 in the intestine, but without major shifts in the microbiome. In conclusion, Bl-04 treatment may influence the host response against H1N1 in a murine challenge model; however, further studies are required to elucidate the mechanism of action.
Funder
Danisco Sweeteners Oy
Subject
Virology,Microbiology (medical),Microbiology
Reference66 articles.
1. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: A systematic analysis for the Global Burden of Disease Study 2010;Lozano;Lancet,2012 2. Estimates of global seasonal influenza-associated respiratory mortality: A modelling study;Iuliano;Lancet,2018 3. Lee, K.H., Gordon, A., Shedden, K., Kuan, G., Ng, S., Balmaseda, A., and Foxman, B. (2019). The respiratory microbiome and susceptibility to influenza virus infection. PLoS ONE, 14. 4. Association Between the Respiratory Microbiome and Susceptibility to Influenza Virus Infection;Tsang;Clin. Infect. Dis.,2020 5. Van Kerkhove, M.D., Vandemaele, K.A.H., Shinde, V., Jaramillo-Gutierrez, G., Koukounari, A., Donnelly, C.A., Carlino, L.O., Owen, R., Paterson, B., and Pelletier, L. (2011). Risk Factors for Severe Outcomes following 2009 Influenza A (H1N1) Infection: A Global Pooled Analysis. PLoS Med., 8.
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