Pheno- and Genotyping of Three Novel Bacteriophage Genera That Target a Wheat Phyllosphere Sphingomonas Genus
-
Published:2023-07-18
Issue:7
Volume:11
Page:1831
-
ISSN:2076-2607
-
Container-title:Microorganisms
-
language:en
-
Short-container-title:Microorganisms
Author:
Riber Leise1ORCID, Carstens Alexander Byth1, Dougherty Peter Erdmann1, Roy Chayan1, Willenbücher Katharina2, Hille Frank2ORCID, Franz Charles M. A. P.2, Hansen Lars Hestbjerg1
Affiliation:
1. Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg, Denmark 2. Department of Microbiology and Biotechnology, Max Rubner-Institut, Hermann-Weigmann-Strasse 1, 24103 Kiel, Germany
Abstract
Bacteriophages are viral agents that infect and replicate within bacterial cells. Despite the increasing importance of phage ecology, environmental phages—particularly those targeting phyllosphere-associated bacteria—remain underexplored, and current genomic databases lack high-quality phage genome sequences linked to specific environmentally important bacteria, such as the ubiquitous sphingomonads. Here, we isolated three novel phages from a Danish wastewater treatment facility. Notably, these phages are among the first discovered to target and regulate a Sphingomonas genus within the wheat phyllosphere microbiome. Two of the phages displayed a non-prolate Siphovirus morphotype and demonstrated a narrow host range when tested against additional Sphingomonas strains. Intergenomic studies revealed limited nucleotide sequence similarity within the isolated phage genomes and to publicly available metagenome data of their closest relatives. Particularly intriguing was the limited homology observed between the DNA polymerase encoding genes of the isolated phages and their closest relatives. Based on these findings, we propose three newly identified genera of viruses: Longusvirus carli, Vexovirus birtae, and Molestusvirus kimi, following the latest ICTV binomial nomenclature for virus species. These results contribute to our current understanding of phage genetic diversity in natural environments and hold promising implications for phage applications in phyllosphere microbiome manipulation strategies.
Funder
Novo Nordic Foundation Villum Foundation
Subject
Virology,Microbiology (medical),Microbiology
Reference81 articles.
1. Mann, N.H. (2005). The third age of phage. PLoS Biol., 3. 2. Bacteriophage genomics;Hendrix;Curr. Opin. Microbiol.,2003 3. Bacteriophages: An appraisal of their role in the treatment of bacterial infections;Hanlon;Int. J. Antimicrob. Agents,2007 4. Batinovic, S., Wassef, F., Knowler, S.A., Rice, D.T.F., Stanton, C.R., Rose, J., Tucci, J., Nittami, T., Vinh, A., and Drummond, G.R. (2019). Bacteriophages in Natural and Artificial Environments. Pathogens, 8. 5. Effects of viruses and predators on prokaryotic community composition;Jardillier;Microb. Ecol.,2005
|
|