Characterization of the Bacterial Microbiome in Natural Populations of Barley Stem Gall Midge, Mayetiola hordei, in Morocco
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Published:2023-03-21
Issue:3
Volume:11
Page:797
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ISSN:2076-2607
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Container-title:Microorganisms
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language:en
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Short-container-title:Microorganisms
Author:
Remmal Imane12ORCID, Bel Mokhtar Naima13, Maurady Amal12ORCID, Reda Britel Mohammed1, El Fakhouri Karim4ORCID, Asimakis Elias3ORCID, Tsiamis George3ORCID, Stathopoulou Panagiota3ORCID
Affiliation:
1. Laboratory of Innovative Technologies, National School of Applied Sciences of Tangier, Abdelmalek Essaâdi University, BP 1818 Tanger Principal, Tanger 90000, Morocco 2. Faculty of Sciences and Technology of Tangier, Abdelmalek Essâadi University, Tétouan 93000, Morocco 3. Laboratory of Systems Microbiology and Applied Genomics, Department of Sustainable Agriculture, University of Patras, 30100 Agrinio, Greece 4. AgroBioSciences Program, College for Sustainable Agriculture and Environmental Science, Mohammed VI Polytechnic University, Lot 660, Hay Moulay Rachid, Ben Guerir 43150, Morocco
Abstract
Mayetiola hordei (Kieffer), known as barley stem gall midge, is one of the most destructive barley pests in many areas around the world, inflicting significant qualitative and quantitative damage to crop production. In this study, we investigate the presence of reproductive symbionts, the effect of geographical origin on the bacterial microbiome’s structure, and the diversity associated with natural populations of M. hordei located in four barley-producing areas in Morocco. Wolbachia infection was discovered in 9% of the natural populations using a precise 16S rDNA PCR assay. High-throughput sequencing of the V3-V4 region of the bacterial 16S rRNA gene indicated that the native environments of samples had a substantial environmental impact on the microbiota taxonomic assortment. Briefly, 5 phyla, 7 classes, and 42 genera were identified across all the samples. To our knowledge, this is the first report on the bacterial composition of M. hordei natural populations. The presence of Wolbachia infection may assist in the diagnosis of ideal natural populations, providing a new insight into the employment of Wolbachia in the control of barley midge populations, in the context of the sterile insect technique or other biological control methods.
Funder
European Regional Development Fund of the European Union and Greek national funds
Subject
Virology,Microbiology (medical),Microbiology
Reference125 articles.
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