Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials

Author:

Vanheuverzwijn Jérome1,Maillard Eloise-Eliane1,Mahat Amal1ORCID,Fowler Lee2,Monteyne Daniel3,Bonnaud Leïla4ORCID,Landercy Nicolas4,Hemberg Axel4,Janković Ana5,Meyer Franck1ORCID,Mišković-Stanković Vesna6ORCID,Stevanović Milena5ORCID,Mirica Codruta7,Pérez-Morga David3,Luginbuehl Reto89ORCID,Combes Christèle10ORCID,Furtos Gabriel11ORCID,Fontaine Véronique1ORCID

Affiliation:

1. Microbiology, Bioorganic and Macromolecular Chemistry Unit, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Boulevard du Triomphe, 1050 Brussels, Belgium

2. Applied Materials Science, The Ångström Laboratory, Department of Engineering Sciences, Uppsala University, P.O. Box 534, 75121 Uppsala, Sweden

3. Laboratoire de Parasitologie Moléculaire, Faculté des Sciences & CMMI, Université Libre de Bruxelles (ULB), CP 300. Rue Prof. Jeener & Brachet, 12, 6041 Gosselies, Belgium

4. Center of Innovation and Research in Materials and Polymers, Materia Nova Research Center & University of Mons, 7000 Mons, Belgium

5. Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia

6. Faculty of Ecology and Environmental Protection, University Union-Nikola Tesla, Cara Dusana 62-64, 11158 Belgrade, Serbia

7. Department of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, Victor Babes Street 15, 400012 Cluj-Napoca, Romania

8. Department of Biomedical Material Research, University of Bern, 3008 Bern, Switzerland

9. Blaser Swisslube, 3415 Hasle-Rüegsau, Switzerland

10. Centre Inter-Universitaire de Recherche et d’Ingénierie des Matériaux, CIRIMAT, Toulouse INP, Université Toulouse 3 Paul Sabatier, CNRS, Université de Toulouse, 4 allée Emile Monso, BP44362, CEDEX 4, 31030 Toulouse, France

11. Department of Dental Materials, Institute of Research in Chemistry, Babes-Bolyai University-Raluca Ripan, Fantanele Street 30, 400294 Cluj-Napoca, Romania

Abstract

Medical implants have improved the quality of life of many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims of this research were to develop an easy, robust, quantitative assay to assess surface antimicrobial activities, especially the anti-nascent biofilm activity, and to identify control surfaces, allowing for international comparisons. Using new antimicrobial assays to assess the inhibition of nascent biofilm during persistent contact or after transient contact with bacteria, we show that the 5 cent Euro coin or other metal-based antibacterial coins can be used as positive controls, as more than 4 log reduction on bacterial survival was observed when using either S. aureus or P. aeruginosa as targets. The methods and controls described here could be useful to develop an easy, flexible and standardizable assay to assess relevant antimicrobial activities of new implant materials developed by industries and academics.

Funder

European Union’s Horizon 2020 research and innovation program

Publisher

MDPI AG

Subject

Virology,Microbiology (medical),Microbiology

Reference28 articles.

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5. Kanematsu, H., and Barry, D.M. (2020). Formation and Control of Biofilm in Various Environments, Springer. [1st ed.].

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