Effects of NaHCO3 Stress on Black Locust (Robinia pseudoacacia L.) Physiology, Biochemistry, and Rhizosphere Bacterial Communities

Abstract

Soil salinization has become an ecological and environmental problem that cannot be ignored. Tetraploid black locust (Robinia pseudoacacia L.) is a leguminous tree with characteristics of drought and saline-alkali tolerance. Rhizosphere bacteria are the primary functional microorganisms within the plant root system, and they play a crucial role in regulating plant growth and enhancing stress tolerance. However, there is still a lack of research on the effect of saline-alkali stress on the bacterial community structure in the rhizosphere of black locusts. In this study, we applied 0, 50, 100, and 150 mM NaHCO3 stress to diploid (2×) and tetraploid (4×) black locusts for 16 days. We used 16S rDNA sequencing to investigate the changes in the rhizosphere bacterial communities. Furthermore, we evaluated soil enzyme activity and plant physiological characteristics to explore the response of rhizosphere bacteria to NaHCO3 stress. The results demonstrated that the 4× plant exhibited superior alkali resistance compared to its 2× plant counterpart under NaHCO3 stress. Simultaneously, it was observed that low concentrations of NaHCO3 stress notably increased the abundance of rhizosphere bacteria in both plant types, while reducing their diversity. The impact of stress on the rhizosphere bacterial community weakened as the stress concentration increased. The application of NaHCO3 stress caused a significant change in the composition of the bacterial community in the rhizosphere. Additionally, alkaline salt stress influences the diversity of rhizosphere bacterial communities, which are linked to soil enzyme activities. These data will help us better understand the relationship between the dominant rhizosphere bacterial community and black locust. They will also provide a reference for further improving the alkali resistance of black locust by enhancing the soil bacterial community.