Variability in Cold Tolerance of Food and Clinical Listeria monocytogenes Isolates
-
Published:2022-12-26
Issue:1
Volume:11
Page:65
-
ISSN:2076-2607
-
Container-title:Microorganisms
-
language:en
-
Short-container-title:Microorganisms
Author:
Myintzaw PeterORCID, Pennone VincenzoORCID, McAuliffe OliviaORCID, Begley Máire, Callanan Michael
Abstract
The aim of this study was to investigate the level of strain variability amongst food and clinical Listeria monocytogenes isolates growing at low temperatures (4 and 7 °C) in both laboratory media and real food matrices. Isolates (n = 150) grown in laboratory media demonstrated a large variation in growth profiles measured using optical density. Overall, it was noted that clinical isolates exhibited a significantly higher growth rate (p ≤ 0.05) at 7 °C than the other isolates. Analysis of variance (ANOVA) tests of isolates grouped using Multi Locus Sequence Typing (MLST) revealed that clonal complex 18 (CC18) isolates were significantly (p ≤ 0.05) faster growing at 4 °C than other CC-type isolates while CC101, CC18, CC8, CC37 and CC14 were faster growing than other CC types at 7 °C. Euclidean distance and Ward method-based hierarchical clustering of mean growth rates classified 33.33% of isolates as faster growing. Fast and slow growing representative isolates were selected from the cluster analysis and growth rates were determined using plate count data in laboratory media and model food matrices. In agreement with the optical density experiments, CC18 isolates were faster and CC121 isolates were slower than other CC types in laboratory media, UHT milk and fish pie. The same trend was observed in chocolate milk but the differences were not statistically significant. Moreover, pan-genome analysis (Scoary) of isolate genome sequences only identified six genes of unknown function associated with increased cold tolerance while failing to identify any known cold tolerance genes. Overall, an association that was consistent in laboratory media and real food matrices was demonstrated between isolate CC type and increased cold tolerance.
Funder
Department of Agriculture, Food and the Marine under the Food Institutional Research Measure
Subject
Virology,Microbiology (medical),Microbiology
Reference45 articles.
1. European Food Safety Authority, and European Centre for Disease Prevention and Control (2021). The European Union One Health 2020 Zoonoses Report. EFSA J., 19, e06971. 2. Centers for Disease Control and Prevention (CDC) (2022, June 03). Table 1. Annual Reported Cases of Notifiable Diseases and Rates per 100,000, United States, Excluding U.S. Territories and Non-U.S. Residents, Available online: https://wonder.cdc.gov/nndss/static/2019/annual/2019-table1.html. 3. Subtyping of Bacterial Foodborne Pathogens;Wiedmann;Nutr. Rev.,2002 4. Ragon, M., Wirth, T., Hollandt, F., Lavenir, R., Lecuit, M., Le Monnier, A., and Brisse, S. (2008). A New Perspective on Listeria monocytogenes Evolution. PLoS Pathog., 4. 5. Wareth, G., Linde, J., Hammer, P., Splettstoesser, W., Pletz, M., Neubauer, H., and Sprague, L. (2021). Molecular Characterization of German Acinetobacter baumannii Isolates and Multilocus Sequence Typing (MLST) Analysis Based on WGS Reveals Novel STs. Pathogens, 10.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|