Molecular Detection and Genotyping of Theileria spp. in Deer (Cervidae) in Korea

Author:

Chung Chang Uk12,Lee Haeseung3,Seo Min-Goo1ORCID,Lee Seung-Hun4ORCID,Kim Kyoo-Tae1ORCID,Nazim Kaifa5,Song Jung-Sun6,Bae Dong Hwa6,Rhee Man Hee1ORCID,Kwon Oh-Deog1,Kwak Dongmi17ORCID

Affiliation:

1. College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Daegu, Republic of Korea

2. Wild Animal Rescue Center, Andong 36605, Gyeongbuk, Republic of Korea

3. Veterinary Epidemiology Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Gyeongbuk, Republic of Korea

4. College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Chungbuk, Republic of Korea

5. Department of Veterinary Parasitology, Khalsa College of Veterinary & Animal Sciences, Amritsar 143002, Punjab, India

6. Department of Veterinary Nursing Science, Yeungjin University, Daegu 41527, Daegu, Republic of Korea

7. Cardiovascular Research Institute, Kyungpook National University, Daegu 41944, Daegu, Republic of Korea

Abstract

Major clinical symptoms of Theileria infection include fever, anemia, anorexia, jaundice, and decreased milk production. Although several studies have been conducted on tick-borne pathogens, including Theileria in Korea, only a few have focused on Theileria infection in deer, including the Korean water deer. Blood samples from 160 deer were collected and subjected to DNA extraction and polymerase chain reaction (PCR). Next, PCR-positive samples were sequenced and analyzed by constructing a phylogenetic tree. The results showed that the overall infection rate of Theileria was 8.1% (13/160). Infection rates of 100% were observed in the northern and southern regions. However, the study’s limitation was its small sample size, wherein five and one samples were analyzed from the northern and southern regions, respectively. The central region exhibited the lowest infection rate of 2.9% (4/140). Infection rates also differed based on seasons, with the highest (18.4%, 9/49) being observed in spring, followed by that in summer (8.9%, 4/45). However, no infection was observed during autumn and winter. A phylogenetic analysis indicated that the PCR-positive samples contained Theileria luwenshuni, which usually infects small ruminants, such as goats and sheep.

Funder

National Research Foundation, Republic of Korea

Publisher

MDPI AG

Subject

Virology,Microbiology (medical),Microbiology

Reference54 articles.

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