GC-MS Analysis, Antibacterial, and Anticancer Activities of Hibiscus sabdariffa L. Methanolic Extract: In Vitro and In Silico Studies
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Published:2023-06-16
Issue:6
Volume:11
Page:1601
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ISSN:2076-2607
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Container-title:Microorganisms
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language:en
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Short-container-title:Microorganisms
Author:
Sehim Amira E.1ORCID, Amin Basma H.2ORCID, Yosri Mohammed2ORCID, Salama Hanaa M.3, Alkhalifah Dalal Hussien4, Alwaili Maha Abdullah4, Abd Elghaffar Rasha Y.1
Affiliation:
1. Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13518, Egypt 2. The Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo 11787, Egypt 3. Department of Chemistry, Faculty of Science, Port Said University, Port Said 42521, Egypt 4. Department of Biology, Collage of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Abstract
The emergence of bacteria that are resistant to several antibiotics has represented a serious hazard to human health globally. Bioactive metabolites from medicinal plants have a wide spectrum of therapeutic possibilities against resistant bacteria. Therefore, this study was performed to investigate the antibacterial efficacy of various extracts of three medicinal plants as Salvia officinalis L., Ziziphus spina-christi L., and Hibiscus sabdariffa L. against pathogenic Gram-negative Enterobacter cloacae (ATCC13047), Pseudomonas aeruginosa (RCMB008001), Escherichia coli (RCMB004001), and Gram-positive Staphylococcus aureus (ATCC 25923), bacteria using the agar-well diffusion method. Results revealed that, out of the three examined plant extracts, the methanol extract of H. sabdariffa L. was the most effective against all tested bacteria. The highest growth inhibition (39.6 ± 0.20 mm) was recorded against E. coli. Additionally, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the methanol extract of H. sabdariffa were detected in the case of all tested bacteria. Moreover, an antibiotic susceptibility test revealed that all tested bacteria showed multidrug resistance (MDR). While 50% of tested bacteria were sensitive and 50% were intermediately sensitive to piperacillin/tazobactam (TZP) based on the inhibition zone but still less than the extract. Synergistic assay demonstrated the promising role of using a combination of H. sabdariffa L. and (TZP) against tested bacteria. A surface investigation using a scanning electron microscope of the E. coli treated with TZP, extract, or a combination of the two revealed extremely considerable bacterial cell death. In addition, H. sabdariffa L. has a promising anticancer role versus Caco-2 cells with IC50 of 17.51 ± 0.07 µg/mL and minimal cytotoxicity upon testing versus Vero cells with CC50 of 165.24 ± 0.89 µg/mL. Flow cytometric analysis confirmed that H. sabdariffa extract significantly increased the apoptotic rate of Caco-2-treated cells compared to the untreated group. Furthermore, GC-MS analysis confirmed the existence of various bioactive components in the methanol hibiscus extract. Utilizing molecular docking with the MOE-Dock tool, binding interactions between n-Hexadecanoic acid, hexadecanoic acid-methyl ester, and oleic acid, 3-hydroxypropyl ester were evaluated against the target crystal structures of E. coli (MenB) (PDB ID:3T88) and the structure of cyclophilin of a colon cancer cell line (PDB ID: 2HQ6). The observed results provide insight into how molecular modeling methods might inhibit the tested substances, which may have applications in the treatment of E. coli and colon cancer. Thus, H. sabdariffa methanol extract is a promising candidate to be further investigated for developing alternative natural therapies for infection treatment.
Funder
Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
Subject
Virology,Microbiology (medical),Microbiology
Reference71 articles.
1. Advances in novel antibiotics to treat multidrug-resistant gram-negative bacterial infections;Matlock;Intern. Emerg. Med.,2021 2. General principles of antibiotic resistance in bacteria;Martinez;Drug Discov. Today Technol.,2014 3. Cilloniz, C., Martin-Loeches, I., Garcia-Vidal, C., San Jose, A., and Torres, A. (2016). Microbial Etiology of Pneumonia: Epidemiology, Diagnosis and Resistance Patterns. Int. J. Mol. Sci., 17. 4. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance;Magiorakos;Clin. Microbiol. Infect.,2012 5. Multidrug-resistant, extensively drug-resistant and Pandrug-resistant bacteria and antimicrobial therapy in combination;Shamsuzzaman;Bangladesh J. Med. Microbiol.,2015
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