SW16-7, a Novel Ackermannviridae Bacteriophage with Highly Effective Lytic Activity Targets Salmonella enterica Serovar Weltevreden
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Published:2023-08-15
Issue:8
Volume:11
Page:2090
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ISSN:2076-2607
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Container-title:Microorganisms
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language:en
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Short-container-title:Microorganisms
Author:
Xu Jialiang1, Li Jia1, Yan Yi1ORCID, Han Pengjun2, Tong Yigang2ORCID, Li Xu1
Affiliation:
1. China Food Flavor and Nutrition Health Innovation Center, Beijing Technology and Business University, Beijing 100048, China 2. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
Abstract
Salmonella enterica serovar Weltevreden is a foodborne pathogen commonly transmitted through fresh vegetables and seafood. In this study, a lytic phage, SW16-7, was isolated from medical sewage, demonstrating high infectivity against S. Weltevreden, S. London, S. Meleagridis, and S. Give of Group O:3. In vitro inhibition assays revealed its effective antibacterial effect for up to 12 h. Moreover, analysis using the Comprehensive Antibiotic Resistance Database (CARD) and the Virulence Factor Database (VFDB) showed that SW16-7’s genome does not contain any virulence factors or antibiotic resistance genes, indicating its potential as a promising biocontrol agent against S. Weltevreden. Additionally, a TSP gene cluster was identified in SW16-7’s genome, with TSP1 and TSP2 showing a high similarity to lysogenic phages ε15 and ε34, respectively, in the C-terminal region. The whole-genome phylogenetic analysis classified SW16-7 within the Ackermannviridae family and indicated a close relationship with Agtrevirus, which is consistent with the ANI results.
Funder
Beijing Outstanding Young Scientist Program
Subject
Virology,Microbiology (medical),Microbiology
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