Affiliation:
1. Laboratory of Biological Engineering, Guamá Science and Technology Park, Belém 66075-750, Brazil
2. Laboratory of Genomics and Bioinformatics, Center of Genomics and Systems Biology, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, Brazil
3. Laboratory of Medical and Human Genetics, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, Brazil
Abstract
Mangroves provide a unique ecological environment for complex microbial communities, which play important roles in biogeochemical cycles, such as those for carbon, sulfur, and nitrogen. Microbial diversity analyses of these ecosystems help us understand the changes caused by external influences. Amazonian mangroves occupy an area of 9000 km2, corresponding to 70% of the mangroves in Brazil, on which studies of microbial biodiversity are extremely scarce. The present study aimed to determine changes in microbial community structure along the PA-458 highway, which fragmented a mangrove zone. Mangrove samples were collected from three zones, (i) degraded, (ii) in the process of recovery, and (iii) preserved. Total DNA was extracted and submitted for 16S rDNA amplification and sequencing on an MiSeq platform. Subsequently, reads were processed for quality control and biodiversity analyses. The most abundant phyla were Proteobacteria, Firmicutes, and Bacteroidetes in all three mangrove locations, but in significantly different proportions. We observed a considerable reduction in diversity in the degraded zone. Important genera involved in sulfur, carbon, and nitrogen metabolism were absent or dramatically reduced in this zone. Our results show that human impact in the mangrove areas, caused by the construction of the PA-458 highway, has resulted in a loss of biodiversity.
Funder
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Subject
Virology,Microbiology (medical),Microbiology
Reference54 articles.
1. Potential Microbial Diversity in Mangrove Ecosystems:A Review;Sahoo;Indian J. Mar. Sci.,2009
2. The Role of Sediment Microorganisms in the Productivity, Conservation, and Rehabilitation of Mangrove Ecosystems: An Overview;Holguin;Biol. Fertil. Soils,2001
3. Spatial Patterns of Soil Attributes and Components in a Mangrove System in Southeast Brazil (São Paulo);Ferreira;J. Soils Sediments,2010
4. Expanding Perspectives of Soil Nitrification to Include Ammonia-Oxidizing Archaea and Comammox Bacteria;Lu;Soil. Sci. Soc. Am. J.,2020
5. Tian, J., Ge, F., Zhang, D., Deng, S., and Liu, X. (2021). Roles of Phosphate Solubilizing Microorganisms from Managing Soil Phosphorus Deficiency to Mediating Biogeochemical P Cycle. Biology, 10.
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献