PeVL1 Novel Elicitor Protein, from Verticillium lecanii 2, Enhances Systemic Resistance against Rice Leaf Roller (Marasmia ruralis Wlk.) in Rice (Oryza sativa L.)

Abstract

The hazardous pest known as rice leaf roller (Marasmia ruralis Wlk.) (Lepidoptera: Pyralidae), which undermines rice (Oryza sativa L.) output globally, folds the leaves of the rice plant. Protein elicitors are thought to be biological elements that causes the rice to become resistant to herbivores. The potential for biocontrol of the emerging elicitor protein evaluated from Verticillium lecanii 2 (PeVL1) was evaluated against M. ruralis. To assess the impact of PeVL1 on immature development, survival, and lifetime, four different PeVL1 concentrations were allocated. Electrical penetration graphs (EPGs) against M. ruralis were used to evaluate adult reproductive efficiency and the interaction between the pest and the pathogen. Furthermore, the characterization of active substances in PeVL1 with multi-acting entomopathogenic effects looked into the direct interactions of PeVL1 with temperature and climatic change in rice (O. sativa) plants. PeVL1 treatments reduced the population increase of second and third generation M. ruralis compared to controls. In a test of host selection, M. ruralis colonized control plants more quickly than PeVL1-treated O. sativa plants. PeVL1 concentrations prolonged the M. ruralis larval stage. Similar to fecundity, PeVL1-treated seedlings produced fewer offspring than control seedlings. On PeVL1-treated leaves, trichomes and wax production created an unfavorable habitat for M. ruralis. PeVL1 changed the surface structure of the leaves, which inhibited colonization and decreased M. ruralis reproduction. The activation of pathways was another aspect of systemic defense activities including jasmonic acid (JA), salicylic acid (SA), and ethylene (ET). Based on these results against M. ruralis, the use of PeVL1 in the agroecosystem with integrated pest management and biocontrol seems appropriate. Our research provides a novel insight into a cutting-edge biocontrol method utilizing V. lecanii 2.