Insights from the Endophytic Fungi in Amphisphaeria (Sordariomycetes): A. orixae sp. nov. from Orixa japonica and Its Secondary Metabolites

Author:

Wang Xiaojie12,Wanasinghe Dhanushka N.3ORCID,Zhang Jingyi4ORCID,Ma Jian4,Zhou Peifeng12,Zhang Lijuan4,Lu Yongzhong4,Zhang Zhen2

Affiliation:

1. School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China

2. Guizhou Academy of Testing and Analysis, Guizhou Academy of Sciences, Guiyang 550014, China

3. Centre for Mountain Futures, Kunming Institute of Botany, Chinese Academy of Sciences, Honghe County 654400, China

4. School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, China

Abstract

Endophytic fungi are a remarkably diverse group of microorganisms that have imperceptible associations with their hosts for at least a part of their life cycle. The enormous biological diversity and the capability of producing bioactive secondary metabolites such as alkaloids, terpenoids, and polyketides have attracted the attention of different scientific communities, resulting in numerous investigations on these fungal endophytes. During our surveys of plant-root-based fungi in the mountain areas of Qingzhen, Guizhou Province, several isolates of endophytic fungi were identified. In this study, a novel endophytic fungus was discovered in the roots of a medicinal plant (Orixa japonica) in Southern China and introduced as a new species (Amphisphaeria orixae) based on morphological evidence and molecular phylogenetic analysis (combined ITS and LSU sequence data). To the best of our knowledge, A. orixae is the first reported endophyte as well as the first hyphomycetous asexual morph in Amphisphaeria. A new isocoumarin, (R)-4,6,8-trihydroxy-5-methylisochroman-1-one (1), and 12 known compounds (2–13) were isolated from the rice fermentation products of this fungus. Using 1D- and 2D-NMR, mass spectrometry, and ECD studies, their structures were identified. The antitumor activity of these compounds was tested. Unfortunately, none of the compounds tested showed significant antitumor activity.

Funder

Youth Science and Technology Talent Development Project from the Guizhou Provincial Department of Education

Guizhou Province high-level talent innovation and entrepreneurship merit funding project

academic emerging project of Guizhou Institute of Technology

Publisher

MDPI AG

Subject

Virology,Microbiology (medical),Microbiology

Reference46 articles.

1. DNA based identification and phylogenetic characterisation of endophytic and saprobic fungi from Antidesma madagascariense, a medicinal plant in Mauritius;Jeewon;J. Mycol.,2013

2. Isolation of endophytic fungi and screening of Huperzine A–producing fungus from Huperzia serrata in Vietnam;Sci. Rep.,2019

3. Plant Probiotics–Endophytes pivotal to plant health;Pandey;Microbiol. Res.,2022

4. Research advances on endophytic fungi and their bioactive metabolites;Wang;Bioprocess. Biosyst. Eng.,2022

5. Rat small intestine muscle relaxation alkaloids from Orixa japonica leaves;Funayama;Biol. Pharm. Bull.,2001

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