Red LED Light Improves Pepper (Capsicum annuum L.) Seed Radicle Emergence and Growth through the Modulation of Aquaporins, Hormone Homeostasis, and Metabolite Remobilization
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Published:2023-03-01
Issue:5
Volume:24
Page:4779
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Zaghdoud Chokri1ORCID, Ollio Irene23ORCID, Solano Cristóbal J.4ORCID, Ochoa Jesús23ORCID, Suardiaz Juan4ORCID, Fernández Juan A.23ORCID, Martínez Ballesta María del Carmen23ORCID
Affiliation:
1. Bureau de Transfert de Technologie (BuTT), Université de Gafsa, Gafsa 2112, Tunisia 2. Ingeniería Agronómica, Technical University of Cartagena, Paseo Alfonso XIII 48, E-30203 Cartagena, Spain 3. Recursos Fitogenéticos, Instituto de Biotecnología Vegetal, Edificio I+D+i, E-30202 Cartagena, Spain 4. División of Innovation in Telematic Systems and Electronic Technology (DINTEL), Technical University of Cartagena, Campus Muralla del Mar, s/n, E-30202 Cartagena, Spain
Abstract
Red LED light (R LED) is an efficient tool to improve seed germination and plant growth under controlled environments since it is more readily absorbed by photoreceptors’ phytochromes compared to other wavelengths of the spectrum. In this work, the effect of R LED on the radicle emergence and growth (Phase III of germination) of pepper seeds was evaluated. Thus, the impact of R LED on water transport through different intrinsic membrane proteins, via aquaporin (AQP) isoforms, was determined. In addition, the remobilization of distinct metabolites such as amino acids, sugars, organic acids, and hormones was analysed. R LED induced a higher germination speed index, regulated by an increased water uptake. PIP2;3 and PIP2;5 aquaporin isoforms were highly expressed and could contribute to a faster and more effective hydration of embryo tissues, leading to a reduction of the germination time. By contrast, TIP1;7, TIP1;8, TIP3;1 and TIP3;2 gene expressions were reduced in R LED-treated seeds, pointing to a lower need for protein remobilization. NIP4;5 and XIP1;1 were also involved in radicle growth but their role needs to be elucidated. In addition, R LED induced changes in amino acids and organic acids as well as sugars. Therefore, an advanced metabolome oriented to a higher energetic metabolism was observed, conditioning better seed germination performance together with a rapid water flux.
Funder
Polytechnic University of Cartagena
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference128 articles.
1. Feng, H., Nemzer, B., and DeVries, J.W. (2019). Sprouted Grains: Nutritional Value, Production and Applications, AACC International Press. 2. Germination-Still a mystery;Nonogaki;Plant Sci.,2010 3. Evaluation of benzyl-butyl phthalate induced germination and early growth vulnerability to barley seedlings (Hordeum vulgare L.);Kumari;Indian J. Ecol.,2018 4. Bewley, J.D., Bradford, K.J., Hilhorst, H.W.M., and Nonogaki, H. (2013). Seeds: Physiology of Development, Germination and Dormancy, Springer. [3rd ed.]. 5. Alves Junior, C., de Oliveira Vitoriano, J., da Silva, D.L.S., de Lima Farias, M., and de Lima Dantas, N.B. (2016). Water uptake mechanism and germination of Erythrina velutina seeds treated with atmospheric plasma. Sci. Rep., 6.
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