Morphological and Physio-Chemical Responses to PEG-Induced Water Stress in Vanilla planifolia and V. pompona Hybrids
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Published:2023-02-28
Issue:5
Volume:24
Page:4690
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Barreda-Castillo José Martín1ORCID, Monribot-Villanueva Juan L.2ORCID, Velázquez-Rosas Noé1ORCID, Bayman Paul3ORCID, Guerrero-Analco José A.2ORCID, Menchaca-García Rebeca Alicia1ORCID
Affiliation:
1. Centro de Investigaciones Tropicales, Universidad Veracruzana, Xalapa 91090, Veracruz, Mexico 2. Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Clúster Científico y Tecnológico BioMimic®, Xalapa 91073, Veracruz, Mexico 3. Department of Biology, University of Puerto Rico, Río Piedras, San Juan 00925, Puerto Rico
Abstract
Vanilla planifolia is an orchid of cultural and economic value. However, its cultivation in many tropical countries is threatened by water stress. In contrast, V. pompona is a species that is tolerant of prolonged periods of drought. Due to the need for plants’ resistant to water stress, the use of hybrids of these two species is considered. Therefore, the objective of this study was to evaluate the morphological and physio-chemical responses of in vitro vanilla seedlings of the parental genotype V. planifolia, and the hybrids V. planifolia × V. pompona and V. pompona × V. planifolia, which were then exposed over five weeks to polyethylene glycol-induced water stress (−0.49 mPa). Stem and root length, relative growth rate, number of leaves and roots, stomatal conductance, specific leaf area, and leaf water content were determined. Metabolites potentially associated with the response to water stress were identified in leaves, through untargeted and targeted metabolomics. Both hybrids exhibited a smaller decrease in the morphophysiological responses compared to V. planifolia and exhibited an enrichment of metabolites such as carbohydrates, amino acids, purines, phenols, and organic acids. Hybrids of these two species are considered as a potential alternative to the traditional cultivation of vanilla to face drought in a global warming scenario.
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference105 articles.
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