Extraction Potential of Lolium perenne L. (Perennial Rye Grass) for Metals in Landfill Soil: Its Tolerance and Defense Strategies

Abstract

Landfill sites open and close frequently throughout the world, taking over a significant amount of land and leaving it contaminated and unavailable to the surrounding population for use. Different forms of remediation methods have been employed to rehabilitate contaminated land to a state that poses less of a threat to the environment. Phytoremediation is one of the remediation techniques that has proven to be effective, economical and easier to implement compared to other methods. The main aim of this study was to explore the potential use of Lolium perenne L. to remediate and restore metal-contaminated landfill soil and determine its stress tolerance mechanism(s). The metal uptake, determined using inductively coupled plasma-optical emission spectroscopy (ICP-OES) and inductively coupled plasma-mass spectroscopy (ICP-MS), revealed that Lolium perenne accumulate a higher amount of metals in the roots than in leaves, which was further confirmed by the translocation factor (TF) values of all of the metals that were below 1, ranging between 0.2 and 0.8, while Cu, Cr and Pb had a bioaccumulation factor (BCF) > 1. This confirms that L. perenne is capable of absorbing metals into the root matrix but might restrict further movement into other parts of the plant as a defense mechanism against metal toxicity. In response to metal-induced stress, L. perenne displayed an increase in enzyme activity of superoxide dismutase, glutathione S-transferase, peroxidase and amylases in plants grown in landfill soil. Peroxidases displayed the highest level of enzyme activity, while total amylolytic activity had the most significant increase in activity over time. Although not a hyperaccumulator, L. perenne is a potential candidate for the phytoremediation of landfill soil and the phytostabilization of Cu, Cr and Pb.