Uncovering the Underlying Mechanisms Blocking Replication of Bluetongue Virus Serotype 26 (BTV-26) in Culicoides Cells

Author:

Monsion Baptiste1ORCID,Mohd Jaafar Fauziah1ORCID,Mertens Peter P. C.2,Attoui Houssam1ORCID

Affiliation:

1. UMR1161 VIROLOGIE, INRAE, Ecole Nationale Vétérinaire d’Alfort, ANSES, Université Paris-Est, F-94700 Maisons-Alfort, France

2. One Virology, The Wolfson Centre for Global Virus Research, School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, Leicestershire, UK

Abstract

At least 12 serotypes of ‘atypical’ bluetongue virus (BTV-25 to BTV-36) have been identified to date. These atypical serotypes fail to infect/replicate in Culicoides-derived cell lines and/or adult Culicoides vectors and hence can no longer be transmitted by these vectors. They appear to be horizontally transmitted from infected to in-contact ruminants, although the route(s) of infection remain to be identified. Viral genome segments 1, 2 and 3 (Seg-1, Seg2 and Seg-3) of BTV-26 were identified as involved in blocking virus replication in KC cells. We have developed Culicoides-specific expression plasmids, which we used in transfected insect cells to assess the stability of viral mRNAs and protein expression from full-length open reading frames of Seg-1, -2 and -3 of BTV-1 (a Culicoides-vectored BTV) or BTV-26. Our results indicate that the blocked replication of BTV-26 in KC cells is not due to an RNAi response, which would lead to rapid degradation of viral mRNAs. A combination of degradation/poor expression and/or modification of the proteins encoded by these segments appears to drive the failure of BTV-26 core/whole virus-particles to assemble and replicate effectively in Culicoides cells.

Funder

European Commission’s H2020 PALE Blu Project

Publisher

MDPI AG

Subject

Molecular Biology,Biochemistry

Reference53 articles.

1. Attoui, H., Mohd Jaafar, F., and Mertens, P.P.C. (2018, January 24–28). In the Reovirales a new taxonomic order: Families Sedoreoviridae and Spinareoviridae. Proceedings of the 13th International dsRNA Virus Symposium, Houffalize, Belgium.

2. ICTV Virus Taxonomy Profile: Sedoreoviridae 2022;Matthijnssens;J. Gen. Virol.,2022

3. Mohd Jaafar, F., Belhouchet, M., Belaganahalli, M., Tesh, R.B., Mertens, P.P., and Attoui, H. (2014). Full-genome characterisation of Orungo, Lebombo and Changuinola viruses provides evidence for co-evolution of orbiviruses with their arthropod vectors. PLoS ONE, 9.

4. Genetic characterization of the tick-borne orbiviruses;Belaganahalli;Viruses,2015

5. Strategies for the sequence determination of viral dsRNA genomes;Attoui;J. Virol. Methods,2000

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3