Involvement of the MGF 110-11L Gene in the African Swine Fever Replication and Virulence

Author:

Tamás Vivien1ORCID,Righi Cecilia2ORCID,Mészáros István1,D’Errico Federica2ORCID,Olasz Ferenc1,Casciari Cristina2,Zádori Zoltán1,Magyar Tibor1ORCID,Petrini Stefano2ORCID,Feliziani Francesco2ORCID

Affiliation:

1. Institute for Veterinary Medical Research, Hungária krt. 21, 1143 Budapest, Hungary

2. Istituto Zooprofilattico Sperimentale Umbria-Marche “Togo Rosati”, Via Gaetano Salvemini, 1, 06126 Perugia, Italy

Abstract

African swine fever (ASF) is a highly lethal hemorrhagic viral disease that causes extensive economic and animal welfare losses in the Eurasian pig (Sus scrofa) population. To date, no effective and safe vaccines have been marketed against ASF. A starting point for vaccine development is using naturally occurring attenuated strains as a vaccine base. Here, we aimed to remove the multigene family (MGF) 110 gene of unknown function from the Lv17/WB/Rie1 genome to improve the usability of the virus as a live-attenuated vaccine, reducing unwanted side effects. The MGF 110-11L gene was deleted using the CRISPR/Cas9 method, and the safety and efficacy of the virus were tested in pigs after isolation. The vaccine candidates administered at high doses showed reduced pathogenicity compared to the parental strain and induced immunity in vaccinated animals, although several mild clinical signs were observed. Although Lv17/WB/Rie1/d110-11L cannot be used as a vaccine in its current form, it was encouraging that the undesirable side effects of Lv17/WB/Rie1 at high doses can be reduced by additional mutations without a significant reduction in its protective capacity.

Funder

European Union’s Horizon 2020 Research and Innovation Program

Publisher

MDPI AG

Subject

Pharmacology (medical),Infectious Diseases,Drug Discovery,Pharmacology,Immunology

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