Immunogenicity and Efficacy of Monovalent and Bivalent Formulations of a Virus-Like Particle Vaccine against SARS-CoV-2
Author:
Resch Matthew D.ORCID, Wen Ke, Mazboudi Ryan, Mulhall Maasz Hannah, Persaud Mirjana, Garvey Kaitlyn, Gallardo Leslie, Gottlieb PaulORCID, Alimova Aleksandra, Khayat RezaORCID, Morales Jorge, Bielefeldt-Ohmann Helle, Bowen Richard A., Galarza Jose M.
Abstract
Virus-like particles (VLPs) offer great potential as a safe and effective vaccine platform against SARS-CoV-2, the causative agent of COVID-19. Here, we show that SARS-CoV-2 VLPs can be generated by expression of the four viral structural proteins in a mammalian expression system. Immunization of mice with a monovalent VLP vaccine elicited a potent humoral response, showing neutralizing activity against multiple variants of SARS-CoV-2. Subsequent immunogenicity and efficacy studies were performed in the Golden Syrian hamster model, which closely resembles the pathology and progression of COVID-19 in humans. Hamsters immunized with a bivalent VLP vaccine were significantly protected from infection with the Beta or Delta variant of SARS-CoV-2. Vaccinated hamsters showed reduced viral load, shedding, replication, and pathology in the respiratory tract. Immunized hamsters also showed variable levels of cross-neutralizing activity against the Omicron variant. Overall, the VLP vaccine elicited robust protective efficacy against SARS-CoV-2. These promising results warrant further study of multivalent VLP vaccines in Phase I clinical trials in humans.
Funder
National Institute of Allergy and Infectious Diseases U.S. Department of Defense
Subject
Pharmacology (medical),Infectious Diseases,Drug Discovery,Pharmacology,Immunology
Reference60 articles.
1. (2022, October 26). WHO Coronavirus (COVID-19) Dashboard. Available online: https://covid19.who.int. 2. García-Montero, C., Fraile-Martínez, O., Bravo, C., Torres-Carranza, D., Sanchez-Trujillo, L., Gómez-Lahoz, A.M., Guijarro, L.G., García-Honduvilla, N., Asúnsolo, A., and Bujan, J. (2021). An Updated Review of SARS-CoV-2 Vaccines and the Importance of Effective Vaccination Programs in Pandemic Times. Vaccines, 9. 3. Eroglu, B., Nuwarda, R.F., Ramzan, I., and Kayser, V. (2022). A Narrative Review of COVID-19 Vaccines. Vaccines, 10. 4. Holder, J. (2021). Tracking Coronavirus Vaccinations around the World. N. Y. Times, 30. 5. Ssentongo, P., Ssentongo, A.E., Voleti, N., Groff, D., Sun, A., Ba, D.M., Nunez, J., Parent, L.J., Chinchilli, V.M., and Paules, C.I. (2022). SARS-CoV-2 Vaccine Effectiveness against Infection, Symptomatic and Severe COVID-19: A Systematic Review and Meta-Analysis. BMC Infect. Dis., 22.
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|