Development of Immersion and Oral Bivalent Nanovaccines for Streptococcosis and Columnaris Disease Prevention in Fry and Fingerling Asian Seabass (Lates calcarifer) Nursery Farms

Author:

Meachasompop Pakapon12,Bunnoy Anurak12ORCID,Keaswejjareansuk Wisawat3ORCID,Dechbumroong Piroonrat3,Namdee Katawut3ORCID,Srisapoome Prapansak12ORCID

Affiliation:

1. Laboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 50 Paholayothin Rd., Ladyao, Chatuchak, Bangkok 10900, Thailand

2. Center of Excellence in Aquatic Animal Health Management, Faculty of Fisheries, Kasetsart University, 50 Paholayothin Rd., Ladyao, Chatuchak, Bangkok 10900, Thailand

3. National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, Thailand

Abstract

In the present study, chitosan-based bivalent nanovaccines of S. iniae and F. covae were administered by immersion vaccination at 30 and 40 days after hatching (DAH), and the third vaccination was orally administered by feeding at 50 DAH. ELISA revealed that the levels of total IgM and specific IgM to S. iniae and F. covae were significantly elevated in all vaccinated groups at 10, 20, and 30 days after vaccination (DAV). A qRT-PCR analysis of immune-related genes revealed significantly higher IgT expression in the vaccinated groups compared to the control group, as revealed by 44–100-fold changes in the vaccinated groups compared to the control (p < 0.001) at every tested time point after vaccination. All vaccinated groups expressed IgM, MHCIIα, and TCRα at significantly higher levels than the control group at 10 and/or 20 DAV (p < 0.05). In the S. iniae challenge tests, the survival of vaccinated groups ranged from 62.15 ± 2.11 to 75.70 ± 3.36%, which significantly differed from that of the control group (44.44 ± 1.92%). Similarly, all vaccinated groups showed higher survival rates of 68.89 ± 3.85 to 77.78 ± 5.09% during F. covae challenge than the control groups (50.00 ± 3.33%) (p < 0.05).

Funder

Thai Union Feedmill Public Company Limited

National Science and Technology Development Agency

Publisher

MDPI AG

Subject

Pharmacology (medical),Infectious Diseases,Drug Discovery,Pharmacology,Immunology

Reference51 articles.

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4. Columnaris disease in fish: A review with emphasis on bacterium-host interactions;Declercq;Vet. Res.,2013

5. Streptococcus iniae infection in cultured Asian sea bass (Lates calcarifer) and red tilapia (Oreochromis sp.) in southern Thailand;Suanyuk;Songklanakarin J. Sci. Technol.,2010

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