Affiliation:
1. College of Veterinary Medicine, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China
2. College of Food Science and Engineering, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China
3. Basic Medical College, Qingdao University, No. 308 Ningxia Road, Qingdao 266071, China
Abstract
Considering that the strip method is simple and convenient for users, a Europium nanosphere-based time-resolved fluorescent immunochromatographic assay (TRFICA) for the rapid screening of 4,4′-dinitrocarbanilide (DNC) was developed to improve the performance of strip assays. After optimization, TRFICA showed IC50, the limit of detection, and cut-off values of 0.4, 0.07, and 5.0 ng mL−1, respectively. No significant cross-reactivity (CR < 0.1%) with 15 DNC analogs was observed in the developed method. TRFICA was validated for DNC detection in spiked chicken homogenates, and recoveries ranged from 77.3% to 92.7%, with coefficients of variation of <14.9%. Moreover, the time needed for the detection procedure, including the sample pre-treatment, was less than 30 min for TRFICA, which had never been achieved before in other immunoassays. The newly developed strip test is a rapid, sensitive, quantitative, and cost-effective on-site screening technique for DNC analysis in chicken muscle.
Funder
Natural Science Foundation of Shandong Province
Postdoctoral Innovation Project of Shandong Province
Postdoctoral Fund of Qingdao, the High-Level Talent Fund of Qingdao Agricultural University
Scientific and Technological Projects of Qingdao
College Students’ Innovative Entrepreneurial Training Plan Program
Subject
Clinical Biochemistry,General Medicine,Analytical Chemistry,Biotechnology,Instrumentation,Biomedical Engineering,Engineering (miscellaneous)