Fe3O4@Au Core–Shell Magnetic Nanoparticles for the Rapid Analysis of E. coli O157:H7 in an Electrochemical Immunoassay-Reference-Cited by-同舟云学术

Fe3O4@Au Core–Shell Magnetic Nanoparticles for the Rapid Analysis of E. coli O157:H7 in an Electrochemical Immunoassay

Published:2023-05-22 Issue:5 Volume:13 Page:567
ISSN:2079-6374
Container-title:Biosensors
language:en
Short-container-title:Biosensors

Author:

Bazsefidpar Shayesteh¹^ORCID,Freitas Maria²^ORCID,Pereira Clara R.³^ORCID,Gutiérrez Gemma⁴^ORCID,Serrano-Pertierra Esther¹^ORCID,Nouws Henri P. A.²^ORCID,Matos María⁴^ORCID,Delerue-Matos Cristina²^ORCID,Blanco-López María Carmen¹^ORCID

Affiliation:

1. Department of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006 Oviedo, Spain

2. REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4249-015 Porto, Portugal

3. REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, 4169-007 Porto, Portugal

4. Department of Chemical and Environmental Engineering & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006 Oviedo, Spain

Abstract

Escherichia coli (E. coli) O157:H7 is a pathogenic bacterium that causes serious toxic effects in the human gastrointestinal tract. In this paper, a method for its effective analytical control in a milk sample was developed. To perform rapid (1 h) and accurate analysis, monodisperse Fe3O4@Au magnetic nanoparticles were synthesized and used in an electrochemical sandwich-type magnetic immunoassay. Screen-printed carbon electrodes (SPCE) were used as transducers, and electrochemical detection was performed by chronoamperometry using a secondary horseradish peroxidase-labeled antibody and 3,3′,5,5′-tetramethylbenzidine. This magnetic assay was used to determine the E. coli O157:H7 strain in the linear range from 20 to 2 × 106 CFU/mL, with a limit of detection of 20 CFU/mL. The selectivity of the assay was tested using Listeria monocytogenes p60 protein, and the applicability of the assay was assessed by analyzing a commercial milk sample, demonstrating the usefulness of the synthesized nanoparticles in the developed magnetic immunoassay.

Funder

European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie

Consejería de Educación y Ciencia del Principado de Asturias

Portuguese national funds

FCT for the Individual Call to Scientific Employment Stimulus contracts

Publisher

MDPI AG

Subject

Clinical Biochemistry,General Medicine,Analytical Chemistry,Biotechnology,Instrumentation,Biomedical Engineering,Engineering (miscellaneous)

Link

https://www.mdpi.com/2079-6374/13/5/567/pdf

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