Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and Microtissues
Author:
Sánchez-Porras David123ORCID, Durand-Herrera Daniel14, Carmona Ramón5, Blanco-Elices Cristina12ORCID, Garzón Ingrid12, Pozzobon Michela67, San Martín Sebastián8ORCID, Alaminos Miguel12ORCID, García-García Óscar Darío12ORCID, Chato-Astrain Jesús12ORCID, Carriel Víctor12ORCID
Affiliation:
1. Tissue Engineering Group, Department of Histology, Faculty of Medicine, Universidad de Granada, 18016 Granada, Spain 2. Instituto de Investigación Biosanitaria ibs.GRANADA, 18012 Granada, Spain 3. Doctoral Program in Biomedicine, Doctoral School, Universidad de Granada, 18016 Granada, Spain 4. Facultad de Odontología, Universidad Michoacana de San Nicolás de Hidalgo (UMSNH), Morelia 58010, Mexico 5. Department of Cell Biology, Faculty of Sciences, Universidad de Granada, 18071 Granada, Spain 6. Department of Women and Children’s Health, University of Padova, 35129 Padova, Italy 7. Corso Stati Uniti 4, Institute of Pediatric Research Città della Speranza, 35127 Padova, Italy 8. Centro de Investigaciones Biomédicas, Escuela de Medicina, Facultad de Medicina, Universidad de Valparaíso, Valparaíso 2520000, Chile
Abstract
Wharton’s jelly stem cells (WJSC) from the human umbilical cord (UC) are one of the most promising mesenchymal stem cells (MSC) in tissue engineering (TE) and advanced therapies. The cell niche is a key element for both, MSC and fully differentiated tissues, to preserve their unique features. The basement membrane (BM) is an essential structure during embryonic development and in adult tissues. Epithelial BMs are well-known, but similar structures are present in other histological structures, such as in peripheral nerve fibers, myocytes or chondrocytes. Previous studies suggest the expression of some BM molecules within the Wharton’s Jelly (WJ) of UC, but the distribution pattern and full expression profile of these molecules have not been yet elucidated. In this sense, the aim of this histological study was to evaluate the expression of main BM molecules within the WJ, cultured WJSC and during WJSC microtissue (WJSC-MT) formation process. Results confirmed the presence of a pericellular matrix composed by the main BM molecules—collagens (IV, VII), HSPG2, agrin, laminin and nidogen—around the WJSC within UC. Additionally, ex vivo studies demonstrated the synthesis of these BM molecules, except agrin, especially during WJSC-MT formation process. The WJSC capability to synthesize main BM molecules could offer new alternatives for the generation of biomimetic-engineered substitutes where these molecules are particularly needed.
Funder
Spanish “Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica” (I+D+i) of the Spanish Ministry of Science and Innovation FEDER funds Consejería de Salud y Familias, Junta de Andalucía, Spain Universidad de Granada Consejería de Transformación Económica, Industria Conocimiento y Universidades, Junta de Andalucía European Union Consejería de Transformación Económica, Industria, Conocimiento y Universidades, Junta de Andalucía
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