Intracellular Fate of Sub-Toxic Concentration of Functionalized Selenium Nanoparticles in Aggressive Prostate Cancer Cells

Author:

Bissardon Caroline1ORCID,Proux Olivier2ORCID,Gazze Salvatore Andrea3,Filhol Odile4ORCID,Toubhans Benoît3,Sauzéat Lucie5ORCID,Bouchet Sylvain5,Lewis Aled R.3,Maffeis Thierry3ORCID,Hazemann Jean-Louis6ORCID,Bayat Sam1ORCID,Cloetens Peter7,Conlan R. Steven3ORCID,Charlet Laurent8,Bohic Sylvain17ORCID

Affiliation:

1. Université Grenoble Alpes, Inserm, UA7, Synchrotron Radiation for Biomedicine (STROBE), 38400 Grenoble, France

2. OSUG, UAR 832 CNRS, Université Grenoble Alpes, IRD, INRAe, Météo-France, 38041 Grenoble, France

3. Swansea University Medical School, Swansea University, Swansea SA2 8PP, UK

4. Laboratoire de Biologie et Biotechnologies pour la Santé, IRIG-DS, Inserm U1292, CEA, Université Grenoble-Alpes, 38054 Grenoble, France

5. Institute of Biogeochemistry and Pollutant Dynamics, ETH Zurich, CH-8092 Zurich, Switzerland

6. Institut Néel CNRS-UGA, 25 Avenue des Martyrs, 38042 Grenoble, France

7. ESRF—The European Synchrotron Radiation Facility, 38043 Grenoble, France

8. ISTerre, Université Grenoble Alpes, 38041 Grenoble, France

Abstract

Selenium 0 (Se0) is a powerful anti-proliferative agent in cancer research. We investigated the impact of sub-toxic concentrations of Se0 functionalized nanoparticles (SeNPs) on prostate cancer PC-3 cells and determined their intracellular localization and fate. An in-depth characterization of functionalized selenium nanoparticles composition is proposed to certify that no chemical bias relative to synthesis issues might have impacted the study. Selenium is an extremely diluted element in the biological environment and therefore requires high-performance techniques with a very low detection limit and high spatial resolution for intracellular imaging. This was explored with state-of-the-art techniques, but also with cryopreparation to preserve the chemical and structural integrity of the cells for spatially resolved and speciation techniques. Monodisperse solutions of SeNPs capped with bovine serum albumin (BSA) were shown to slow down the migration capacity of aggressive prostate cancer cells compared to polydisperse solutions of SeNPs capped with chitosan. BSA coating could prevent interactions between the reactive surface of the nanoparticles and the plasma membrane, mitigating the generation of reactive oxygen species. The intracellular localization showed interaction with mitochondria and also a localization in the lysosome-related organelle. The SeNPs-BSA localization in mitochondria constitute a possible explanation for our result showing a very significant dampening of the PC-3 cell proliferation capabilities. The purpose of the use of sublethal compound concentrations was to limit adverse effects resulting from high cell death to best evaluate some cellular changes and the fate of these SeNPs on PC-3. Our findings provide new insight to further study the various mechanisms of cytotoxicity of SeNPs.

Funder

INCA

Publisher

MDPI AG

Subject

General Materials Science,General Chemical Engineering

Reference61 articles.

1. The importance of selenium to human health;Rayman;Lancet,2000

2. Selenium biochemistry and its role for human health;Roman;Metallomics,2014

3. Selenium in human health and disease;Bao;Antioxid. Redox Signal.,2011

4. Apoptosis is a Critical Cellular Event in Cancer Chemoprevention and Chemotherapy by Selenium Compounds;Sinha;Curr. Cancer Drug Targets,2005

5. Chemical form of selenium, critical metabolites, and cancer prevention;Ip;Cancer Res.,1991

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3